摘要

       移动遗传元件 (MGE) 在环境中不同微生物之间转移抗生素抗性基因 (ARG) 方面发挥着关键作用。本研究旨在探讨鸡粪 (CM) 和牛粪 (BM) 堆肥过程中 MGE 的归宿，以评估 ARG 的水平转移风险。结果表明，CM堆肥过程中MGEs的去除效率显着高于BM堆肥过程，因为CM堆肥过程中MGEs的潜在宿主细菌被大量消除。同时，这些潜在宿主细菌群落受 pH、NH4+、NO3- 和总 N 的显着影响，可用于调节宿主细菌群落以在堆肥过程中去除 MGE。投影追踪回归进一步证实，堆肥可以有效降低 ARGs 的水平转移风险，特别是对于 CM 堆肥。这些结果确定了宿主细菌群落在不同动物粪便堆肥过程中 MGE 去除中的关键作用。

       Mobile genetic elements (MGEs) play critical roles in transferring antibiotic resistance genes (ARGs) among different microorganisms in the environment. This study aimed to explore the fate of MGEs during chicken manure (CM) and bovine manure (BM) composting to assess horizontal transfer risks of ARGs. The results showed that the removal efficiency of MGEs during CM composting was significantly higher than that during BM composting, because the potential host bacteria of MGEs were eliminated largely during CM composting. Meanwhile, these potential host bacterial communities are significantly influenced by pH, NH4+, NO3− and total N, which can be used to regulate host bacterial communities to remove MGEs during composting. Projection pursuit regression further confirmed that composting can effectively reduce the horizontal transfer risk of ARGs, especially for CM composting. These results identified the critical roles of host bacterial communities in MGEs removal during composting of different animal manures.

https://www.sciencedirect.com/science/article/abs/pii/S0269749119301101