摘要

       为了全面调查污水处理厂活性污泥中的四环素耐药性，采用454焦磷酸测序和Illumina高通量测序检测不同浓度四环素培养污泥中潜在的四环素耐药菌（TRB）和抗生素耐药基因（ARGs）。 16S rRNA 基因的焦磷酸测序显示四环素处理极大地影响了污泥的细菌群落结构。由 Sulfuritalea、Armatimonas、Prosthecobacter、Hyphomicrobium、Azonexus、Longilinea、Paracoccus、Novosphingobium 和 Rhodobacter 组成的九个属被确定为污泥中潜在的 TRB。 qPCR、分子克隆和宏基因组分析的结果一致表明，四环素处理可以增加tet基因的丰度和多样性，但降低非四环素ARG的发生率和多样性，尤其是磺胺抗性基因sul2。聚类分析表明，亚抑制浓度（5 mg/L）的四环素处理对细菌群落组成产生更大的影响，这可能是造成 ARGs 丰度变化的原因。本研究表明联合使用454焦磷酸测序和Illumina高通量测序可以有效地探索环境中的ARB和ARGs，未来的研究应包括深入研究污水中微生物群落、ARGs和抗生素之间的关系。处理厂 (STP) 污泥。查看全文

       In order to comprehensively investigate tetracycline resistance in activated sludge of sewage treatment plants, 454 pyrosequencing and Illumina high-throughput sequencing were used to detect potential tetracycline resistant bacteria (TRB) and antibiotic resistance genes (ARGs) in sludge cultured with different concentrations of tetracycline. Pyrosequencing of 16S rRNA gene revealed that tetracycline treatment greatly affected the bacterial community structure of the sludge. Nine genera consisting of Sulfuritalea, Armatimonas, Prosthecobacter, Hyphomicrobium, Azonexus, Longilinea, Paracoccus, Novosphingobium and Rhodobacter were identified as potential TRB in the sludge. Results of qPCR, molecular cloning and metagenomic analysis consistently indicated that tetracycline treatment could increase both the abundance and diversity of the tet genes, but decreased the occurrence and diversity of non-tetracycline ARG, especially sulfonamide resistance gene sul2. Cluster analysis showed that tetracycline treatment at subinhibitory concentrations (5 mg/L) was found to pose greater effects on the bacterial community composition, which may be responsible for the variations of the ARGs abundance. This study indicated that joint use of 454 pyrosequencing and Illumina high-throughput sequencing can be effectively used to explore ARB and ARGs in the environment, and future studies should include an in-depth investigation of the relationship between microbial community, ARGs and antibiotics in sewage treatment plant (STP) sludge. View Full-Text

https://www.mdpi.com/1422-0067/15/6/10083