摘要

       本研究确定了 14 个常见抗生素抗性基因 (ARG) 和两个整合子在厌氧消化猪粪 52 天和添加不同量的锌期间的累积沼气、甲烷含量和绝对丰度 (AA)。 与不添加锌的对照（CK）相比，添加125 mg L-1（L）和1250 mg L-1（H）锌的累积沼气分别增加了51.2％和56.0％，但没有显着差异 L 和 H 之间。与 CK 相比，除 tetW 和 tetC 外，本研究中检测到的所有其他 ARG 在 L 和 H 反应器中均有所增加。 然而，低浓度的锌（L 反应器）导致 AD 产品中 ARG 的 AA 增加更多。 冗余分析表明，NO3-N和生物锌显着解释了基因的变化，分别占环境因子总变异的60.9%和20.3%。

       This study determined the accumulated biogas, methane content, and absolute abundances (AAs) of 14 common antibiotic resistance genes (ARGs) and two integrons during the anaerobic digestion of swine manure for 52 days with different amounts of added zinc. The accumulated biogas increased by 51.2% and 56.0% with 125 mg L–1 (L) and 1250 mg L–1 (H) zinc, respectively, compared with the control with no added zinc (CK), but there was no significant difference between L and H. Compared with CK, excluding tetW and tetC, all the other ARGs detected in this study increased in the L and H reactors. However, the low concentration of zinc (L reactor) caused greater increases in the AAs of ARGs in the AD products. Redundancy analysis showed that NO3-N and bio-zinc significantly explained the changes in genes, where they accounted for 60.9% and 20.3% of the total variation in the environmental factors, respectively.

https://www.sciencedirect.com/science/article/abs/pii/S0960852417311264