摘要

       非城市地区畜牧业与产业混业发展是我国非常普遍的现象。非城市污水处理系统中抗生素抗性基因（ARGs）的分布尚未引起足够的重视。在本研究中，11 个四环素抗性基因（tetA、tetB、tetC、tetE、tetG、tetL、tetM、tetO、tetQ、tetS 和 tetX）、4 个 AmpC β-内酰胺酶基因（EBC、MOX、FOX 和 CIT）和四个重对广州市4个不同污水源、不同处理工艺的非城市污水厂进行了金属（Cu、Zn、Cd和Pb）检测分析。结果表明，tetA和tetC是最普遍的四环素抗性基因，检测频率为85%，EBC是最普遍的AmpCβ-内酰胺酶基因，检测频率为75%。在所有样品中，四环素抗性基因的相对丰度比 AmpC β-内酰胺酶基因的相对丰度高约 1.6 个数量级。 A/O 是非城市污水厂接收工业或农业废水的最有效工艺。沉淀是从液相中消除 ARG 的最关键过程。大多数 ARG 是在剩余污泥中而不是流出物中携带的。发现 tet 基因与 Zn (r = 0.881, p < 0.01) 之间存在显着相关性，其次是 AmpC 基因与 Cu (r = 0.847, p < 0.01)、tet 基因与 Cu (r = 0.714, p < 0.05) ）。因此，非城市地区污水处理系统中ARGs的污染与重金属共同污染应引起更多关注。

       The mixed development of animal husbandry and industry in non-urban areas is a very common phenomenon in my country. The distribution of antibiotic resistance genes (ARGs) in non-urban sewage treatment systems has not attracted enough attention. In this study, 11 tetracycline resistance genes (tetA, tetB, tetC, tetE, tetG, tetL, tetM, tetO, tetQ, tetS and tetX), 4 AmpC β-lactamase genes (EBC, MOX, FOX And CIT) and four heavy metals (Cu, Zn, Cd and Pb) detection and analysis in 4 non-urban sewage plants with different sewage sources and different treatment processes in Guangzhou. The results showed that tetA and tetC are the most common tetracycline resistance genes with a detection frequency of 85%, and EBC is the most common AmpCβ-lactamase gene with a detection frequency of 75%. In all samples, the relative abundance of the tetracycline resistance gene was about 1.6 orders of magnitude higher than the relative abundance of the AmpC β-lactamase gene. A/O is the most effective process for non-urban sewage plants to receive industrial or agricultural wastewater. Precipitation is the most critical process to eliminate ARG from the liquid phase. Most ARG is carried in the remaining sludge rather than in the effluent. It was found that there was a significant correlation between tet gene and Zn (r = 0.881, p <0.01), followed by AmpC gene and Cu (r = 0.847, p <0.01), tet gene and Cu (r = 0.714, p <0.05) ) ). Therefore, the co-pollution of ARGs and heavy metals in sewage treatment systems in non-urban areas should cause more attention.

https://www.sciencedirect.com/science/article/abs/pii/S0045653516317398