摘要

       肠道微生物组是抗生素抗性基因 (ARG) 的重要储存库。迫切需要有效的方法来管理肠道抵抗组以对抗抗生素耐药性威胁。在这项研究中，我们展示了以肠道微生物群为目标的饮食干预，将肠道细菌的主要发酵从蛋白质转变为碳水化合物，显着降低了肥胖儿童的肠道抵抗力并缓解了代谢综合征。在~2 × 106 个微生物基因的非冗余宏基因组基因目录中，在 131 种基因类型中鉴定出 399 个 ARG，并赋予对 47 种抗生素的抗性。干预后肠道抵抗组的丰富度和多样性均显着降低。 399 个 ARG 中共有 201 个携带在 120 个共丰度基因组 (CAG) 中，这些基因组直接从干预前和干预后样本的基因目录中分类。干预显着减少了克雷伯氏菌、肠杆菌和埃希氏菌中的几种 CAG，它们是多种抗性基因类型的主要中心。因此，饮食干预可能成为减少肠道抵抗组的潜在有效方法

       The gut microbiome is an important repository of antibiotic resistance genes (ARG). There is an urgent need for effective methods to manage the intestinal resistance group to combat the threat of antibiotic resistance. In this study, we demonstrated a diet intervention targeting the gut microbiota, which converts the main fermentation of gut bacteria from protein to carbohydrate, significantly reducing the intestinal resistance of obese children and alleviating metabolic synthesis Levy. In the non-redundant metagenomic gene catalog of ~2 × 106 microbial genes, 399 ARGs were identified among 131 gene types and conferred resistance to 47 antibiotics. After the intervention, the abundance and diversity of the intestinal resistance group were significantly reduced. A total of 201 of the 399 ARGs are carried in 120 common abundance genomes (CAGs), which are directly classified from the gene catalog of samples before and after intervention. The intervention significantly reduced several CAGs in Klebsiella, Enterobacter and Escherichia, which are the main centers of multiple resistance gene types. Therefore, dietary intervention may be a potentially effective method to reduce the intestinal resistance group 

https://www.nature.com/articles/srep24030