摘要

       尽管在过去十年中红海沿岸开发了创纪录数量的娱乐场所和工业区，但这种环境中的抗生素抗性细菌在很大程度上仍未得到探索。在这项研究中，16S rDNA 测序用于鉴定从红海沿岸、近海和红树林地点收集的 12 个沉积物样本中分离出的细菌。定量 PCR 用于估计样本中基因组 DNA 中抗菌素耐药基因 (ARG) 的数量。共分离出 470 种细菌，分为 137 个不同的物种，其中包括 10 个候选新物种。栖息在红海的特定地点的细菌群落很明显。相对而言，从海岸回收的抗药性分离株更多，近海地区的样本含有最多的多重抗药性细菌。在这项研究中检测到 18 种 ARG，编码对氨基糖苷类、β-内酰胺、磺胺、大环内酯、喹诺酮和四环素抗生素的耐药性。 qnrS、aacC2、ermC 和 blaTEM-1 基因常见于沿海和近海地点。与其他收集的样本相比，在来自海岸的明显人为污染（海滩）样本中发现了相对较高的 ARGs，包括 aacC2 和 aacC3。总之，与其他研究相比，在红海沉积物样本中发现抗微生物分离株相对增加。人为活动可能有助于细菌多样性和 ARG 的增加。

       Although a record number of entertainment venues and industrial areas have been developed along the Red Sea coast in the past decade, antibiotic-resistant bacteria in this environment remain largely unexplored. In this study, 16S rDNA sequencing was used to identify bacteria isolated from 12 sediment samples collected from locations along the Red Sea, offshore, and mangroves. Quantitative PCR is used to estimate the number of antimicrobial resistance genes (ARG) in genomic DNA in a sample. A total of 470 species of bacteria were isolated and divided into 137 different species, including 10 new candidate species. The bacterial communities inhabiting specific locations in the Red Sea are obvious. Relatively speaking, more resistant isolates were recovered from the coast, and samples from offshore areas contained the most multi-drug resistant bacteria. In this study, 18 ARGs were detected, encoding resistance to aminoglycosides, β-lactams, sulfonamides, macrolides, quinolone and tetracycline antibiotics. The qnrS, aacC2, ermC and blaTEM-1 genes are commonly found in coastal and offshore locations. Compared with other collected samples, relatively high ARGs, including aacC2 and aacC3, were found in the apparently anthropogenic pollution (beach) samples from the coast. In summary, compared with other studies, there is a relative increase in antimicrobial isolates found in Red Sea sediment samples. Human activities may contribute to the increase of bacterial diversity and ARG.

https://www.sciencedirect.com/science/article/abs/pii/S0048969719318182