摘要

       为了比较不同工艺配置在不同抗生素水平下的性能和抗生素抗性特征，缺氧/好氧膜生物反应器 (MBR) 1#、MBR2# 和序批式反应器 (SBR) 在相同的操作参数下运行。 MBR1#和SBR在高水平和不断增加的抗生素水平下操作，MBR2#接受恒定和低浓度的抗生素。使用 16S rDNA 基因高通量测序和 qPCR 研究微生物群落和抗生素抗性基因 (ARG)。 90% 以上的青霉素和金霉素被强水解去除，其次是磺胺甲恶唑（69.27%–86.25%）生物降解和诺氟沙星（28.66%–53.86%）吸附。工艺配置对总氮去除的影响较大，而抗生素浓度对总磷去除的影响较大。由于膜组件的保留作用，MBR1#在减少磺胺甲恶唑、诺氟沙星和ARGs方面的表现优于SBR。 MBR 中 ARG 的保留效率随着操作而增加。与添加抗生素前的操作分类单元（OTU）数相比，MBR1#和SBR中的OTU数分别下降了23.7%和28.7%，而MBR2#中的OTU数保持相对稳定。与抗生素浓度相比，工艺配置导致微生物群落的差异更大。该研究提供了对含抗生素废水处理影响因素的深入了解。

       In order to compare the performance and antibiotic resistance characteristics of different process configurations at different levels of antibiotics, anoxic/aerobic membrane bioreactor (MBR) 1#, MBR2# and sequencing batch reactor (SBR) are under the same operating parameters run. MBR1# and SBR operate at high and increasing levels of antibiotics, MBR2# accepts constant and low concentrations of antibiotics. Use 16S rDNA gene high-throughput sequencing and qPCR to study microbial communities and antibiotic resistance genes (ARG). More than 90% of penicillin and chlortetracycline are removed by strong hydrolysis, followed by sulfamethoxazole (69.27%-86.25%) biodegradation and norfloxacin (28.66%-53.86%) adsorption. Process configuration has a greater impact on total nitrogen removal, while antibiotic concentration has a greater impact on total phosphorus removal. Due to the retention of membrane components, MBR1# is better than SBR in reducing sulfamethoxazole, norfloxacin and ARGs. The retention efficiency of ARG in MBR increases with operation. Compared with the number of operational classification units (OTU) before the addition of antibiotics, the number of OTUs in MBR1# and SBR decreased by 23.7% and 28.7%, respectively, while the number of OTUs in MBR2# remained relatively stable. Compared with the concentration of antibiotics, the process configuration leads to greater differences in the microbial community. This study provides an in-depth understanding of the factors affecting the treatment of antibiotic-containing wastewater.

https://www.sciencedirect.com/science/article/abs/pii/S0304389419303346