摘要

       详细研究了抗生素、抗生素抗性细菌 (ARB) 和无细胞抗生素抗性基因 (ARG) 的去除以及 ARB 的微生物群落，以了解它们的命运，并为全面膜的可行性提供有价值的信息生物反应器（MBR）。发现了无细胞 ARG 对接收环境的潜在风险。进水抗生素浓度高可以抑制 MBR 污泥的微生物活性，而由于固体保留时间较长，生物量保留较高，可以保持良好的抗生素去除。大约61.8%~77.5%的抗生素被降解，平均22.5%~38.2%的抗生素被MBR污泥吸附。由于抗生素的化学结构和分配系数，单个抗生素的去除呈现出强烈的差异。气单胞菌对氨苄青霉素和红霉素表现出特定的抗生素耐药性，埃希氏菌成为卡那霉素-ARB和四环素-ARB的优势属，克雷伯菌和拟杆菌是对环丙沙星表现出明显抗生素耐药性的特殊属。发现无细胞 ARG 丰度与 ARB 含量之间存在显着相关性，并且相对较高的流出物无细胞 ARG 丰度促进了 ARB 的增殖和传播。由于无细胞 ARG 的低衰减率和长期持久性，应研究接收环境对消除生态风险和对人类健康的严重威胁的影响。

       The removal of antibiotics, antibiotic resistant bacteria (ARB) and cell-free antibiotic resistance genes (ARG) and the microbial communities of ARBs are studied in detail to understand their fate and provide valuable information on the feasibility of the full-scale membrane biological response器(MBR). The potential risk of cell-free ARG to the receiving environment was discovered. The high concentration of influent antibiotics can inhibit the microbial activity of MBR sludge, and due to the longer solid retention time and the higher biomass retention, good antibiotic removal can be maintained. About 61.8%~77.5% of antibiotics are degraded, and 22.5%~38.2% of antibiotics are adsorbed by MBR sludge on average. Due to the chemical structure and partition coefficient of antibiotics, the removal of individual antibiotics presents a strong difference. Aeromonas shows specific antibiotic resistance to ampicillin and erythromycin, Escherichia becomes the dominant genera of kanamycin-ARB and tetracycline-ARB, and Klebsiella and Bacteroides are resistant to cyclopropyl Floxacin is a special genus that exhibits obvious antibiotic resistance. It is found that there is a significant correlation between the abundance of cell-free ARG and the content of ARB, and the relatively high abundance of cell-free ARG in the effluent promotes the proliferation and spread of ARB. Due to the low attenuation rate and long-term durability of cell-free ARG, the influence of the receiving environment on eliminating ecological risks and serious threats to human health should be studied.

https://www.sciencedirect.com/science/article/abs/pii/S0960852420300948