摘要

       为了研究四环素（TC）和磺胺甲恶唑（SMX）选择压力下微生物群落特征、抗生素抗性基因（ARGs）和生物反应器出水水质的变化，使用了环境相关浓度和高水平TC的序批式反应器（SBRs）和 SMX 浓度（0、5 ppb、50 ppb 和 10 ppm）。化学需氧量 (COD) 和氨氮 (NH4+单键 N) 去除量在 5 和 50 ppb 时没有变化 (p > 0.05)，但在 10 ppm 时显着下降 (p < 0.05)。细胞外聚合物 (EPS) 浓度随着 TC 或 SMX 浓度的增加而显着增加 (p < 0.05)。高通量 16S rRNA 基因测序结果表明，变形菌门、放线菌门和拟杆菌门是污泥样品中含量最高的三个门。放线菌百分比随着 TC 或 SMX 浓度的增加而增加，而变形菌和拟杆菌减少。微生物多样性在 5 ppb 时达到最大值，并随着浓度升高而降低。污泥中总ARGs丰度随着TC或SMX的加入而增加，相对丰度较高的顺序为sul1>tetG>sul2>tetA>intI1>tetS>tetC。 Pearson 相关分析显示大多数 ARG（tetA、tetC、tetG、tetK、tetM、sul1）与 intI1 显着相关（p < 0.01）

       In order to study the changes of microbial community characteristics, antibiotic resistance genes (ARGs) and bioreactor effluent quality under the selection pressure of tetracycline (TC) and sulfamethoxazole (SMX), a sequencing batch method with environmentally relevant concentrations and high levels of TC was used Reactor (SBRs) and SMX concentration (0, 5 ppb, 50 ppb and 10 ppm). The removal of chemical oxygen demand (COD) and ammonia nitrogen (NH4+single bond N) did not change at 5 and 50 ppb (p> 0.05), but decreased significantly at 10 ppm (p <0.05). The concentration of extracellular polymer (EPS) increased significantly as the concentration of TC or SMX increased (p <0.05). The high-throughput 16S rRNA gene sequencing results showed that the Proteobacteria, Actinomycetes and Bacteroides were the three phyla with the highest content in sludge samples. The percentage of actinomycetes increased with the increase of TC or SMX concentration, while Proteobacteria and Bacteroides decreased. The microbial diversity reaches its maximum at 5 ppb and decreases as the concentration increases. The abundance of total ARGs in the sludge increased with the addition of TC or SMX, and the order of relative abundance was sul1>tetG>sul2>tetA>intI1>tetS>tetC. Pearson correlation analysis shows that most ARGs (tetA, tetC, tetG, tetK, tetM, sul1) are significantly related to intI1 (p <0.01)

https://www.sciencedirect.com/science/article/abs/pii/S0048969716314590