摘要

       抗生素抗性基因 (ARG) 是新出现的污染物，在农业和城市活动密集地区的沉积物和其他水生区域中发现的含量较高。然而，关于 ARGs 在自然生态系统中的迁移和衰减的定量数据很少，这对于预测它们在释放到接收水域后的命运至关重要。在这里，我们使用田间规模的中观系统来量化水体中 ARG 衰减率以及 ARG 迁移到外围生物膜中，研究了牛饲养场废水中释放的细菌宿主中四环素抗性基因的命运。将饲养场废水添加到 15 个圆柱形 11.3-m3 中层空间（其中一些具有人工基质），模拟五种不同的接收水条件（一式三份）和六种抗性基因（tet(O)、tet(W)、tet( M)、tet(Q)、tet(B) 和 tet(L)) 和 16S-rRNA 基因监测 14 天。 Mesocosm 处理根据光照、微生物补充剂（通过河水添加）和土霉素 (OTC) 水平而变化。六个基因总和 (tetR) 的一阶水柱消失系数 (kd) 在阳光下总是高于在黑暗中（分别为 -0.72 d-1 和 -0.51 d-1）。然而，水柱 kd 因基因而异（tet(O) < tet(W) < tet(M) < tet(Q)；tet(B) 和 tet(L) 低于检测）和一些基因，特别是 tet(W) )，很容易迁移到生物膜中，这表明不同的基因被单独考虑，外围区室被包括在未来的命运模型中。这项工作为模拟水生系统中的 ARG 命运提供了第一个定量现场数据。

       Antibiotic resistance genes (ARG) are emerging pollutants, found in higher levels in sediments and other aquatic areas in areas where agriculture and urban activities are intensive. However, there are few quantitative data on the migration and attenuation of ARGs in natural ecosystems, which is crucial for predicting their fate after they are released into receiving waters. Here, we used a field-scale meso-system to quantify the rate of ARG decay in water bodies and the migration of ARG into peripheral biofilms to study the fate of tetracycline resistance genes in bacterial hosts released from cattle farm wastewater. The feedlot wastewater is added to 15 cylindrical 11.3-m3 middle spaces (some of which have artificial substrates), simulating five different receiving water conditions (in triplicate) and six resistance genes (tet(O), tet( W), tet(M), tet(Q), tet(B) and tet(L)) and 16S-rRNA gene monitoring for 14 days. Mesocosm treatment varies according to light, microbial supplements (added via river water) and oxytetracycline (OTC) levels. The first-order water column disappearance coefficient (kd) of the sum of six genes (tetR) is always higher in the sun than in the dark (-0.72 d-1 and -0.51 d-1, respectively). However, the water column kd varies from gene to gene (tet(O)

https://pubs.acs.org/doi/abs/10.1021/es800238e