发布者:抗性基因网 时间:2021-09-22 浏览量:576
摘要
填埋的抗生素和金属与其耐药基因的发生有关,其长达十年的浸出发育与动态填埋环境条件有关,特别是氮含量不同,有待研究。在这里,我们从中国五个具有代表性的城市固体废物填埋场抽取了浸出物。抗生素的总浓度(5000 - 50000 ng/L)浸出物中的金属(10 - 60毫克/升)在不同部位之间明显不同,它们只与硫化物和四环素耐药基因(P < 0.05)密切相关。 关于亚型耐药基因的丰度,sul1和ermB是占主导地位的抗生素耐药基因(ARGs)和特克、阿尔斯克、 和梅尔是占主导地位的重金属耐药基因(HMRGs):同时,观察到的这些基因的巨大变异似乎与硝酸盐和pH(P<0.05)等环境因素有关。GeoChip 结果进一步表明,超过 85% 的序列抗逆转录虫共成/HMRG 和氮处理基因,特别是去硝化基因,是由相同的细菌物种,如伪多莫纳斯sp.和杆菌sp.,属于浸出物中的主要植物。这些结果扩展了我们对浸气酸盐中ARG、HMRG和氮处理功能之间的联系的了解。
Antibiotics and metals in landfills are related to the occurrence of drug resistance genes, and their ten-year leaching development is related to dynamic landfill environmental conditions, especially the difference in nitrogen content, which needs to be studied. Here, we extracted extracts from five representative municipal solid waste landfills in China. The total concentration of antibiotics (5000-50000 ng/L) The metals (10-60 mg/L) in the extract are significantly different between different parts, and they are only closely related to sulfide and tetracycline resistance genes (P <0.05) . Regarding the abundance of subtype resistance genes, sul1 and ermB are the dominant antibiotic resistance genes (ARGs) and Turk, Alsk, and Mel are the dominant heavy metal resistance genes (HMRGs): At the same time, The huge variation observed in these genes seems to be related to environmental factors such as nitrate and pH (P<0.05). GeoChip results further show that more than 85% of the sequences of anti-retroworm co-forming/HMRG and nitrogen treatment genes, especially denitrification genes, are derived from the same bacterial species, such as Pseudodomonas sp. and Bacillus sp., belong to The main plant in the extract. These results expand our understanding of the link between ARG, HMRG, and nitrogen treatment functions in leaching salt.
https://www.sciencedirect.com/science/article/abs/pii/S0304389420316757