摘要
       环丙沙星（CIP）与金属离子的共同污染导致CIP迁移率，抗微生物活性和抗生素抗性基因的分布/发展的改变。在这项研究中，研究了5种CIP-Me配合物[Me = Al（III），Co（II），Cu（II），Fe（III），Mg]在两种腐殖物质（HS）存在下的稳定性温度18±2℃和4±1℃7天。最稳定的配合物是CIP-Al，CIP-Cu和CIP-Co，在18℃时的稳定常数（K）分别为35.5±1.4,11.5±1.5和11.7±1.5。在较低温度（4℃）下，稳定常数下降：CIP-Al为1倍，CIP-Co为14倍，CIP-Cu为2倍。腐殖物质的存在降低了配合物的稳定性。在环境中性pH下，水中Fe3 +的化学反应导致稳定性改变。在较低温度和HS存在下CIP-Mg复合物的形成受到限制。在超纯水中，CIP-Me复合物对革兰氏阴性肠杆菌铜绿假单胞菌表现出较高的毒性（介于0.125和0.5μg/ ml之间）。然而，HS的存在将CIP-Me复合物的抗微生物活性降低了至少2倍。革兰氏阳性代表性枯草芽孢杆菌不受金属离子和/或HS的影响。对枯草芽孢杆菌复合物的毒性等同于单独CIP的毒性（MIC =0.25μg/ ml）。这表明了对CIP及其复合物的不同敏感性。

       The co-contamination of ciprofloxacin (CIP) with metal ions results in alteration of CIP mobility, antimicrobial activity and distribution/development of the antibiotic-resistance genes. In this study, the stability of five CIP-Me complexes [Me = Al(III), Co(II), Cu(II), Fe(III), Mg] was investigated in the presence of humic substances (HS) at two temperatures 18 ± 2 °C and 4 ± 1 °C for seven days period. The most stable complexes were CIP-Al, CIP-Cu, and CIP-Co with the stability constants (K) at 18 °C 35.5 ± 1.4 11.5 ± 1.5 and 11.7 ± 1.5 respectively. At lower temperature (4 °C), the stability constants decreased: 1-fold for CIP-Al, 14-fold for CIP-Co and 2-fold for CIP-Cu. The presence of humic substances decreased the stability of complexes. The chemical reactions of Fe3+ in water at circumneutral pH resulted in stability alteration. The formation of CIP-Mg complexes at lower temperatures and in the presence of HS was limited. In ultrapure water, CIP-Me complexes exhibit higher toxicity towards Gram-negative Enterobacter aeruginosa (ranged between 0.125 and 0.5 μg/ml). However, the presence of HS reduced the antimicrobial activity of CIP-Me complexes by at least 2-fold. Gram-positive representative, Bacillus subtilis was not affected by the presence of metal ions and/or HS. The toxicity toward B. subtilis for the complexes was equal to toxicity of CIP alone (MIC = 0.25 μg/ml). This suggested the different susceptibility to CIP and its complexes.

https://www.ncbi.nlm.nih.gov/pubmed/29587236