摘要

人类感染致病性弧菌与污染的海鲜消费有关。在本研究中，我们检查了从埃及零售市场收集的225种甲壳类动物。还检查了来自胃肠炎患者的粪便样品。细菌学和分子检查揭示了来自甲壳类动物的34株（15.1％）副溶血性弧菌和2株（0.9％）霍乱弧菌，而3例（3％）人类样品中鉴定出副溶血性弧菌分离株。毒力相关基因tdh和/或trh分别在甲壳动物和人类样品的5.9％和100％中检测到，而两种霍乱弧菌分离物对ctx和hlyA基因为阳性。抗生素敏感性显示分离菌对所用抗生素的高度耐药性，平均MAR指数为0.77。不同温度下的生物膜形成表明在37°C和25°C时生物膜形成明显高于4°C。频繁监测海产品中的弧菌物种及其抗生素，分子和生物膜特征对于提高海产品安全性至关重要。

Human infection with pathogenic vibrios is associated with contaminated seafood consumption. In the present study, we examined 225 crustaceans collected from retail markets in Egypt. Stool samples from gastroenteritis patients were also examined. Bacteriological and molecular examinations revealed 34 (15.1%) V. parahaemolyticus and 2 (0.9%) V. cholerae from crustaceans, while V. parahaemolyticus isolates were identified in 3 (3%) of the human samples. The virulence-associatedgenes tdh and/or trh were detected in 5.9% and 100% of the crustacean and human samples, respectively, whereas the two V. cholerae isolates were positive for the ctx and hlyA genes. Antibiotic sensitivity revealed high resistance of the isolates to the used antibiotics and an average MAR index of 0.77. Biofilm formation at different temperatures indicated significantly higher biofilm formation at 37 °C and 25 °C compared with 4 °C. Frequent monitoring of seafood for Vibrio species and their antibiotic, molecular and biofilm characteristics is essential to improve seafood safety.

https://www.ncbi.nlm.nih.gov/pubmed/29587179