摘要

       为实现剩余污泥稳定化，并评估剩余污泥的生态环境风险，本研究采用热碱解-中温两相厌氧消化（Thermal-Alkaline Pretreatment-Mesophilic Two Phase Anaerobic Digestion，taMTPAD）工艺处理剩余污泥.考察了热碱解条件及在20、16和10 d 3个不同的总水力停留时间（Total Hydraulic Retention Time，tHRT）下，taMTPAD工艺的运行效果及消化前后抗生素抗性基因（ARGs）的变化.结果表明，对于taMTPAD工艺，当tHRT=10 d时，产酸相的挥发性脂肪酸（VFA）积累量和产甲烷相的日产气量达到最大值，但对ARGs的控制效果最差；tHRT=16 d时反应器的运行效果与tHRT=20 d时接近，但对磺胺类ARGs的控制效果不如tHRT=20 d时；当tHRT=20 d时，挥发性固体（VS）去除率和ARGs的削减效果最好.对比tHRT=10 d时的taMTPAD工艺和MTPAD（两相厌氧消化）工艺，发现对剩余污泥进行热碱预处理能提升产酸相150.32%的VFA产量和产甲烷相89.3%的甲烷产率，但同时热碱解却使得厌氧消化后污泥部分ARGs（sul1、tetO、tetW、tetX）相对丰度回升.研究表明，延长tHRT有利于taMTPAD工艺削减ARGs和VS，而缩短tHRT则有利于产气.

       In order to stabilize the remaining sludge and assess the ecological and environmental risks of the remaining sludge, this study adopted the thermal-alkaline pretreatment-mesophilic two-phase anaerobic digestion (taMTPAD) process to treat the remaining sludge. . The thermal alkaline hydrolysis conditions and the operating effects of the taMTPAD process and the changes of antibiotic resistance genes (ARGs) before and after digestion under three different Total Hydraulic Retention Time (tHRT) of 20, 16 and 10 d were investigated. The results show that for the taMTPAD process, when tHRT=10 d, the accumulation of volatile fatty acids (VFA) in the acid-producing phase and the daily gas production in the methane-producing phase reach the maximum, but the control effect on ARGs is the worst; tHRT=16 The operating effect of the reactor at d is close to that of tHRT=20 d, but the control effect on sulfonamide ARGs is not as good as that of tHRT=20 d; when tHRT=20 d, the removal rate of volatile solids (VS) and the reduction effect of ARGs Best. Comparing the taMTPAD process and the MTPAD (two-phase anaerobic digestion) process when tHRT=10 d, it is found that hot alkali pretreatment of the remaining sludge can increase the VFA output of the acid-producing phase by 150.32% and the methane-producing phase by 89.3%. Methane production rate, but at the same time, thermal alkaline hydrolysis makes the relative abundance of some ARGs (sul1, tetO, tetW, tetX) of the sludge after anaerobic digestion rise. Studies have shown that extending tHRT is beneficial to the taMTPAD process to reduce ARGs and VS, and shorten tHRT It is conducive to gas production.

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