在韩国，从进口肉中分离出20个产超广谱β-内酰胺酶（ESBL）的大肠杆菌菌株。在鸡（14/20）中检测到的大肠杆菌ESBL菌株比猪肉（6/20）和牛肉（0/20）更频繁;在巴西肉类中检测到的最高数字（12/20）。 blaCTX-M基因在来自许多国家的肉类中占优势。来自法国进口猪肉的大肠杆菌产生了blaCTX-M-58酶，这在以前从临床或环境来源的产ESBL细菌中没有证实。此外，在ESBL大肠杆菌分离株中首次发现blaCTX-M-2和blaOXA-1酶在EC12-5分离物中的共存。在40％的ESBL大肠杆菌分离株中发现了罕见的blaCTX-M型blaCTX-M-25。表型敏感性试验表明，大肠杆菌分离株可耐受多达11种抗生素，包括环丙沙星。这是第一次在来自巴西进口的家禽的大肠杆菌分离物中发现了整合子基因盒aacA4-cmlA6-qacEΔ1中的新组合。来自产生CTX-M-94的禽致病谱系的三株大肠杆菌ST117分离物包含fimH，fyuA，iutA，papC，rfc和traT毒力基因，并且不对喹诺酮敏感。这是第一次在从肉制品中分离出的ESBL大肠杆菌菌株中检测到rfc和papG毒力因子。尽管大肠杆菌CC21和CC22分别来自美国和巴西的肉类，但它们在rep-PCR和相同的MLST类型（ST117），表型抗生素抗性模式，整合子基因（ qacEΔ1）和质粒DNA谱。这项研究表明，进口肉类产品可能是韩国产ESBL大肠杆菌菌株的来源。

Twenty extended-spectrum β-lactamase (ESBL)-producing E. coli strains were isolated from imported meat in South Korea. ESBL strains of E. coli were detected in chicken (14/20) more often than in pork (6/20) and beef (0/20); the highest number (12/20) was detected in Brazilian meats. The blaCTX-M genes were predominant in meats from many countries. E. coli from pork imported from France produced the blaCTX-M-58 enzyme, which has never been documented previously in ESBL-producing bacteria from clinical or environmental sources. Additionally, the coexistence of the blaCTX-M-2 and blaOXA-1 enzymes in EC12-5 isolate was found for the first time in an ESBL E. coli isolate. A rare blaCTX-M type, blaCTX-M-25, was found in 40% of ESBL E. coli isolates. Phenotypic susceptibility testing showed that E. coli isolates were resistant to up to eleven antibiotics, including ciprofloxacin. For the first time, a new combination in an integron gene cassette, aacA4-cmlA6-qacEΔ1, was found in an E. coli isolate from poultry imported from Brazil. Three E. coli ST117 isolates, from an avian pathogenic lineage producing CTX-M-94, harbored fimH, fyuA, iutA, papC, rfc, and traT virulence genes and were not susceptible to quinolones. For the first time, rfc and papG virulence factors were detected in ESBL E. coli strains isolated from meat products. Even though E. coli CC21 and CC22 were obtained from meats from the USA and Brazil, respectively, they had a similarity coefficient higher than 99% in rep-PCR and the same MLST type (ST117), phenotypic antibiotic resistance pattern, integron gene (qacEΔ1), and plasmid DNA profile. This study indicates that imported meat products may be a source of ESBL-producing E. coli strains in South Korea.

https://www.ncbi.nlm.nih.gov/pubmed/29580473