摘要

测定了Maiduguri屠宰场屠宰牛和骆驼粪便样品中志贺毒素生产大肠杆菌（STEC）血清群的流行率和分布以及它们对分离株的抗生素抗性概况。9月份记录的流行率最高（24％），更多的STEC分离物来自牛，而不是骆驼。黄牛和骆驼的STEC流行率季节性趋势显着（P <0.05），在湿季中记录的病例数更多。虽然这项研究没有显示来自各种来源的性别的显着影响。本研究记录的血清群为O157，O26，O91，O103和O111。血清群检出率无显着差异（P <0.05）。血清型O26显着（P <0.05）在骆驼和牛中观察到最多的血清群。没有任何STEC分离株对O45血清群检测呈阳性。PCR检测表明，86株STEC分离株中有50株（63.3％）携带stx2基因，34株（43％）携带stx1基因，14株携带stx1和stx2基因（16.3％）。其他检测到的基因包括eae和ehlyA。来自Maiduguri屠宰场的牛和骆驼的STEC O157和非O157分离株中的抗微生物药物抗性非常高，并且STEC分离株对至少一种或多种测试的抗微生物剂具有抗性。还有多重耐药性，最常见的发生模式是氨苄西林/萘啶酸和四环素/甲氧苄啶。但所有79株菌对氯霉素，头孢他啶和头孢曲松敏感;因此，这些药物可能是治疗STEC感染的首选药物。

Prevalence and distribution of Shiga toxin-producing Escherichia coli (STEC) serogroups from the faecal samples of cattle and camels slaughter in Maiduguri abattoir and their antibiotic resistance profile of the isolates were determined. The highest prevalence (24%) was recorded in the month of September and more STEC isolates came from cattle than the camels. There was significant (P < 0.05) seasonal trend in the prevalence of STEC among cattle and camel with more cases recorded during the wet season. Although, the study did not demonstrate significant influence of sex from the various sources. The serogroups recorded in this study were O157, O26, O91, O103 and O111. There was no significant difference (P < 0.05) between the detection rates of serogroups. The serogroup O26 was significantly (P < 0.05) the most observed serogroup in both camels and cattle. None of the STEC isolates tested positive for the O45 serogroup. PCR assays shows that 50 (63.3%) of the 86 STEC isolates carried the stx2 gene, 34 (43%) possessed the stx1 gene, and 14 (16.3%) carried both stx1 and stx2 genes. Othergenes detected include eae and ehlyA. The antimicrobial resistance among the STEC O157 and non-O157 isolates from cattle and camels in Maiduguri abattoir were very high and the STEC isolates were resistant to at least one or more of the antimicrobial agents tested. There was also multidrug resistance with the most frequent occurring patterns been ampicillin/nalidixic acid and tetracycline/trimethoprim. However, all the 79 isolates were sensitive to chloramphenicol, ceftazidime and ceftriaxone; therefore, these drugs could be drugs of choice in the treatment of STEC infections.

https://www.ncbi.nlm.nih.gov/pubmed/29564631