摘要

在这项研究中，建立了微观世界，以确定氮（N）和磷（P）对大肠杆菌多重耐药性和生物膜形成能力的影响。检测生物膜形成相关基因的表达以建立基因型和表型之间的相关性。加入不同浓度的N和P制成一个对照组和四个处理组。玻璃管法用于确定生物膜形成能力。实时PCR用于检测大肠杆菌中六种生物膜形成相关基因的mRNA丰度。没有从对照组中分离出耐药菌株;同时，治疗组的多药耐药率较高。在所有处理组中检测到生物膜相关基因luxS，flhD，fliA，motA和fimH的表达;然而，没有mqsR的表达。 luxS，flhD，fliA，motA和fimH的表达与N，P浓度，以及不同组间多药耐药的表现和持续时间显着相关。总的来说，这项研究的结果表明生物膜形成能力在N和P添加到微观世界中后在大肠杆菌中形成多药耐药性中起关键作用。

In this study, microcosms were established to determine the effect of nitrogen (N) and phosphorus (P) on the multidrugresistance and biofilm-forming abilities of Escherichia coli. The expression of biofilm-formation-related genes was detected to establish correlations between genotype and phenotype. Different concentrations of N and P were added to make one control group and four treatment groups. The glass tube method was used to determine biofilm-forming capabilities. Real-time PCR was used to detect the mRNA abundance of six biofilm-formation-related genes in E. coli. No resistant strains were isolated from the control group; meanwhile, multidrug resistance rates were high in the treatment groups. Expression of the biofilm-associatedgenes luxS, flhD, fliA, motA, and fimH was detected in all treatment groups; however, there was no expression of mqsR. The expression of luxS, flhD, fliA, motA, and fimH significantly correlated with the concentration of N and P, as well as with the appearance and duration of multidrug resistance in different groups. Overall, the results of this study suggest that biofilm-forming ability plays a key role in the formation of multidrug resistance in E. coli after the addition of N and P to a microcosm.

https://www.ncbi.nlm.nih.gov/pubmed/29552003