摘要
      好氧堆肥被认为是一种经济和环境友好的技术，可以防止ARGs和ARBs从粪肥中扩散到农田土壤，对此进行了许多研究。然而，在不同成熟阶段施用粪肥后，ARGs在土壤-蔬菜系统中的传播途径和潜在的生态风险仍不清楚。因此，本研究以叶菜（上海绿）和块茎（胡萝卜）为例，通过盆栽试验，分析了畜禽粪便堆肥对不同土壤蔬菜系统中ARGs和微生物群落的影响。值得注意的是，高度成熟的肥料可以减少土壤和作物中ARGs的污染，并且土壤中的ARGs倾向于向植物根系方向积累。作物的可食用部分通常具有更严重的ARGs生态风险，潜在的致病菌和ARGs可能通过暴露和饮食途径危害人类健康。总之，这项研究为ARGs在粪肥不同成熟阶段的土壤-蔬菜系统中的传播提供了新的证据，并强调了抗生素耐药性从粪肥转移到土壤并最终转移到人类食物链的潜在安全风险。
Abstract
Aerobic composting is considered as an economically as well as environmentally friendly technology that prevents the diffusion of ARGs and ARBs from manure to farmland soil, on which many studies have been conducted. However, the transmission route and potential ecological risks of ARGs in soil-vegetable systems after application of manure at different maturity stages remain unclear. Therefore, through a pot experiment, this study analyzed the effects of manure composted from livestock excrement on ARGs and microbial community in different soil-vegetable systems, taking leafy plants (Shanghai green) and tuber plants (carrot) as examples. It was noted that the highly matured manure could reduce the ARGs contamination in soil and crops, and the ARGs in soil tended to accumulate in the direction of plant roots. The edible part of crops often had a more serious ecological risk of ARGs, and the potential pathogenic bacteria and ARGs could harm human health through exposure and dietary routes. In summary, this study provides new evidence for the transmission of ARGs in soil-vegetable systems with different mature stages of manure and highlights the potential safety risks of the transfer of antibiotic resistance from manure to soil and ultimately to the human food chain.

https://www.sciencedirect.com/science/article/abs/pii/S0301479722016152