摘要
      评估了医院尿液中blaKPC（肺炎克雷伯菌）、blaOXA-50（铜绿假单胞菌）和mecA（金黄色葡萄球菌）基因的缺失，以帮助解决抗生素耐药性细菌的沉默流行病。在电转染和光电转染过程中，使用了一个微流体流通反应器，其MMO阳极和CB/PTFE阴极在50 A m−2下工作。对于blaOXA-50、mecA和blaKPC基因，电转染过程仅实现了几乎可以忽略不计的DNA去除和0.18、0.19和0.71的略对数ARG增量。相反，光电脱毒过程对所有测试的ARB都实现了完全消毒，对blaOXA-50、mecA和blaKPC基因的对数去除率分别为3.70、2.25和0.82。这些结果强调了紫外线与电渗析过程耦合的潜力，不仅可以促进次氯酸盐的形成，还可以促进氯甚至氮自由基的形成，这有助于提高目标ARB及其ARG的消毒效率。
Abstract
The depletion of blaKPC (K. pneumoniae), blaOXA-50 (P. aeruginosa) and mecA (S. aureus) genes from hospital urines is evaluated to contribute to solve the silent pandemic of antibiotic-resistance bacteria. A microfluidic flow-through reactor with MMO anode and CB/PTFE cathode working at 50 A m−2 is employed during electrodisinfection and photo-electrodisinfection processes. The electrodisinfection process only achieves an almost negligible removal of DNA and slightly log ARG increments of 0.18, 0.19 and 0.71 for blaOXA-50, mecA and blaKPC genes, respectively. Conversely, the photo-electrodisinfection process attains the complete disinfection for all ARB tested and logarithmic removals of 3.70, 2.25 and 0.82 for blaOXA-50, mecA and blaKPC genes, respectively. These outcomes emphasize the potential of the UV light coupled to the electrodisinfection process to promote the formation of not only hypochlorite but also chlorine and even nitrogen radicals, which contribute to enhance the disinfection efficiency of the target ARB and their ARGs.

https://www.sciencedirect.com/science/article/pii/S2214714422004792