摘要
      抗生素耐药性基因（ARGs）已经成为对人类健康日益严重的威胁。基于膜的技术已被应用于解决家庭饮用水（HDW）质量的多个问题，包括ARGs。然而，基于膜的HDW净化器的长期运行将不可避免地导致生物污垢，使膜从更清洁变成二次污染源。本研究提出了一种基于群体感应抑制剂（QSI）的策略来控制HDW净化器成品水中的ARGs。选择香兰素（VL）、蔓越莓提取物（CBETs）和单宁酸（TA）三种已报道的QSI，研究它们对典型HDW净化器去除ARGs的影响，并阐明了相应的影响机制。结果表明，ARGs泄漏在没有QSI的情况下发生，但随着VL、CBET和TA的加入而消失。VL的引入使≥3 log ARGs去除的保证率从63.3%提高到70%（对照组），提高到88.9–100%。QSIs对膜过滤去除ARGs的影响机制可归因于直接效应（ARGs宿主的直接失活）和间接效应（调节膜表面的理化性质和生物累积）。这项研究提供了关于QSI诱导的生物污垢的调节机制的数据，并提出了一种通过基于膜的过程控制ARGs的新策略。
Abstract
Antibiotic resistance genes (ARGs) have become an increasing threat to human health. Membrane-based technologies have been applied to address multiple concerns regarding the quality of household drinking water (HDW), including ARGs. However, the long-term operation of membrane-based HDW purifiers would inevitably lead to biofouling, making the membrane turn from cleaner to a secondary pollution source. This study proposed a strategy based on quorum sensing inhibitor (QSI) to control ARGs in the finished water of HDW purifiers. Three reported QSIs, vanillin (VL), cranberry extracts (CBETs), and tannic acid (TA), were selected to investigate their effects on the removal of ARGs by typical HDW purifiers, and the corresponding influencing mechanism was elucidated. The results indicated that ARGs leakage occurred in the absence of QSI but disappeared with the addition of VL, CBETs, and TA. The introduction of VL increased the guarantee rate of ≥ 3 log ARGs removal increased from 63.3 to 70% (control group) to 88.9–100%. The influencing mechanism of QSIs on ARGs removal by membrane filtration can be attributed to direct effect (direct inactivation of the ARGs host) and indirect effects (adjusting the physiochemical properties of the membrane surface and bioaccumulation). This study provides data on the regulatory mechanisms of biofouling induced by QSI and proposes a new strategy for ARGs control by membrane-based processes.

https://www.sciencedirect.com/science/article/abs/pii/S1383586621020566