摘要
      纳米颗粒（NP）在环境中被广泛使用和普遍存在，但它们释放到环境中对抗生素耐药性基因（ARGs）、微生物丰度和群落的影响在很大程度上是未知的。因此，本研究在实验室培养条件下检测了纳米零价铁（nZVI）和氧化锌（nZnO）对沉积物中四环素抗性基因（tet-ARGs）和1类整合素（intI1）的影响。还研究了在tet-ARGs/intI1上NP和四环素（TC）的共存。研究发现，nZVI和nZnO促进了无TC沉积物中tet-ARGs/intI1的丰度，但降低了TC对覆盖TC溶液的沉积物中tet ARGs/intI1的诱导作用。在没有TC的情况下，nZVI、intI1和细菌群落可以直接促进tet-ARGs在nZVI沉积物中的传播，而intI1以及细菌丰度是tet-ARG在nZnO沉积物中传播的最直接原因。对于TC，nZVI和细菌群落可以降低nZVI沉积物中tet-ARGs的丰度，而nZnO和细菌群落则可以直接促进nZnO沉积物中的tet-ARG。最后，这些发现为理解NP在促进和减少环境中ARGs方面的作用提供了宝贵的信息。
Abstract
Nanoparticles (NPs) are widely used and ubiquitous in the environment, but the consequences of their release into the environment on antibiotics resistance genes (ARGs), microbial abundance, and community, are largely unknown. Therefore, this study examined the effect of nano zero-valent iron (nZVI) and zinc oxide (nZnO) on tetracycline resistance genes (tet-ARGs) and class 1 integron (intI1) in sediment under laboratory incubation. The coexistence of NPs and tetracycline (TC) on tet-ARGs/intI1 was also investigated. It was found that nZVI and nZnO promoted tet-ARGs/intI1 abundance in sediment without TC but reduced the inducing effect of TC on tet-ARGs/intI1 in sediment overlaid with TC solution. Without TC, nZVI, intI1, and the bacterial community could directly promote tet-ARGs spread in nZVI sediment, while intI1 and bacterial abundance were the most directly important reasons for tet-ARGs spread in nZnO sediment. With TC, nZVI and bacterial community could reduce tet-ARGs abundance in nZVI sediment, while nZnO and bacterial community could directly promote tet-ARGs in nZnO sediment. Finally, these findings provided valuable information for understanding the role of NPs in promoting and reducing ARGs in the environment.

https://www.mdpi.com/1660-4601/19/15/9405