摘要
      抗生素的不合理使用导致了抗生素耐药性基因（ARGs）在沿海海湾的增殖。关于沿海海湾陆地-海洋相互作用影响下ARGs的季节性分布的报道很少。这项工作研究了在Sishili湾陆地-海洋相互作用影响下ARGs的季节和空间扩散。检测并定量了10个ARGs，包括tetB、tetG、tetX、sul1、sul2、qnrA、qnrB、qnrS、ermF、ermT和1类整合子整合酶基因（intI1）。intI1和大多数ARGs的相对丰度在1×10−7-2拷贝/16S rRNA拷贝的数量级。秋季和夏季的总ARGs丰度远高于其他季节。河口、港口和水产养殖场是海湾中ARGs的重要水库。沿海水中的营养水平与大多数ARGs和intI1呈正相关，表明水质是ARGs及其传播的重要驱动因素。陆地排放和海水分层被证明是沿海海湾ARGs季节性分布的主要驱动因素。春季至夏季，陆地排放和海水分层增强，导致海湾地表水中ARGs急剧增加。
Abstract
The irrational use of antibiotics has given rise to the proliferation of antibiotic resistance genes (ARGs) in coastal bays. There were few reports on the seasonal distribution of ARGs under the influence of land-ocean interaction in coastal bay. This work studied the seasonal and spatial proliferation of ARGs under the influences of land-ocean interaction in the Sishili Bay. Ten ARGs including tetB, tetG, tetX, sul1, sul2, qnrA, qnrB, qnrS, ermF, ermT and class 1 integron-integrase gene (intI1) were detected and quantified. The relative abundances of intI1 and most of ARGs were in orders of magnitude of 1 × 10−7-2 copies/16S rRNA copies. The abundances of total ARGs in autumn and summer were much higher than those in the other seasons. Estuary, port and aquaculture farms were important reservoirs of ARGs in the bay. The nutrient levels in coastal water were positively associated with most of the ARGs and intI1, indicating that the water quality was an important driver of ARGs and their transmission. The land-based discharge and seawater stratification were proved to be the dominant driving factors for the seasonal distribution of ARGs in the coastal bay. The land-based discharge and seawater stratification were enhanced from spring to summer, which led to the sharp increase in ARGs in the surface water of the bay.

https://www.sciencedirect.com/science/article/abs/pii/S0045653522012115