摘要
      近年来，城市雨水径流中的抗生素抗性基因（ARGs）越来越受到关注。然而，关于与径流中运输的道路沉积物相关的ARGs的多样性和分布的数据很少。ARGs多样性调查表明，磺酰胺抗性基因（sul2和sul3）在径流中占总ARGs的61.7%-82.3%。ARGs在颗粒物（PM）中的分布分析表明，tetQ和trbC主要存在于粒径为150–300μm的PM中，但其他ARGs和移动遗传元素（MGE）在粒径<75μm的颗粒物中占主导地位。对潜在宿主的讨论表明，靶基因（ermF、blaOXA1/blaOXA30、ermC、qnrA、sul2、tnpA-01、intI2、tetW、intI1、sul3、trbC）在门水平上与变形杆菌和属水平上与肠杆菌具有最强的从属关系。ARGs分布的效果评估表明，13种ARGs与Pr/PS和Zeta电位呈正相关，导致PM中ARGs越多，尺寸越小（<75μm）。
Abstract
Recently, increasing attention has been paid to antibiotic resistance genes (ARGs) in urban stormwater runoff. However, there were little data on the diversity and distribution of ARGs associated with road sediments transported in runoff. The investigation of ARGs diversity showed that sulfonamide resistance genes (sul2 and sul3) occupied 61.7%–82.3% of total ARGs in runoff. The analysis of ARGs distribution in particulate matter (PM) implied that both tetQ and trbC existed mainly in PM with size of 150–300 μm, but other ARGs and mobile genetic elements (MGEs) were dominant in PM with size <75 μm. The discussion of potential hosts indicated that target genes (ermF, blaOXA1/blaOXA30, ermC, qnrA, sul2, tnpA-01, intI2, tetW, intI1, sul3, trbC) had the strongest subordinate relationship with Proteobacteria at phylum level and Enterobacter at genus level. The effect evaluation of ARGs distribution suggested that 13 kinds of ARGs were positively correlated with Pr/PS and Zeta potential, resulting in the more ARGs in PM with smaller size (<75 μm).

https://www.sciencedirect.com/science/article/abs/pii/S0269749121020522