摘要
      近年来，城市雨水径流中的抗生素抗性基因（ARGs）越来越受到关注。然而，在雨水径流过程中，没有发现关于ARGs从城市下垫面沉积物中浸出的可用文献。在本研究中，选择南京（中国）周围商业公共广场的表层沉积物进行目标ARGs的浸出动力学研究，结果表明，在120-240min的浸出时间内，目标ARGs-解吸的绝对丰度达到平衡，所有解吸效率约为30%，表明在降雨事件期间，随着径流迁移的总ARGs中，浸出ARGs的比例较低。intI1（clinic）、strA、strB、tetM和tetX五种目标ARGs的浸出可以用伪二阶方程更好地描述，而qacEdelta1的浸出可以更好地用伪一阶方程描述，sul1和sul2的浸出都可以用伪一级和伪二阶方程式很好地描述。S/L比、pH值和水温等环境因素的影响表明，目标ARGs的浸出效率随着S/L比和水温的增加而显著提高，但随着pH值的增加而降低。模拟太阳照射和加热后的透射实验表明，大太阳紫外线照射（30W/m2）和高温（40℃）都有利于ARGs的共轭和转化频率。此外，高Cao和中等PAM水平都可以有效阻断ARGs在渗滤液中从下伏表面沉积物中的传输。
Abstract
Recently, increasing attention has been paid to antibiotic resistance genes (ARGs) in urban stormwater runoff. However, no available literature could be found on ARGs leaching from urban underlying surface sediments during stormwater runoff. In this study, surface sediments from commercial public squares around Nanjing (China) was selected for the investigation of target ARGs leaching kinetics, showing that absolute abundances of target ARGs desorption reached at the equilibrium during leaching time of 120-240min with all of the desorption efficiencies about 30%, indicating that there would be low proportion of leaching ARGs in the total ARGs migrating with runoff during rainfall events. Five target ARGs leaching including intI1 (clinic), strA, strB, tetM and tetX can be better described by the pseudo-second-order equation, while qacEdelta1 leaching can be better described by the pseudo-first-order equation, and the leaching for both sul1 and sul2 can be well described by the pseudo-first-order and pseudo-second-order equations. The effects of environmental factors including S/L ratios, pH values and water temperatures indicated that leaching efficiencies of target ARGs enhanced significantly with the increase of S/L ratios and water temperatures, but decreased with the increase of pH values. The transmission experiments after the simulated solar irradiation and heat implied that both large solar UV irradiation (30W/m2) and high temperature (40℃) were conducive to conjugation and transformation frequencies of ARGs. Furthermore, both high Cao and medium PAM levels could be effective for blocking ARGs transmission in the leachate from underlying surface sediments.

https://www.sciencedirect.com/science/article/abs/pii/S0043135422006881