摘要
      粪肥是进入环境的抗生素抗性基因（ARGs）的储存库。本研究研究了添加0%、2.5%、5%和7.5%海泡石（分别为CK、T1、T2和T3）对堆肥过程中ARGs命运的影响。堆肥后，CK和T3中总ARGs的相对丰度分别下降了0.23和0.46 log。10/11 ARGs的RAs在CK中降低，而在T3中均降低。堆肥后，T3中总可移动遗传元素（MGE）的RA降低是CK的1.26倍。细菌群落占ARGs丰度变化的47.93%。网络分析表明，ARGs和MGE共享潜在宿主细菌（PHB），T3通过降低PHB的丰度来控制ARGs的传播。用7.5%海泡石堆肥是降低ARGs扩散风险的有效策略。
Abstract
Manures are storages for antibiotic resistance genes (ARGs) entering the environment. This study investigated the effects of adding sepiolite at 0%, 2.5%, 5%, and 7.5% (CK, T1, T2, and T3, respectively) on the fates of ARGs during composting. The relative abundances (RAs) of the total ARGs in CK and T3 decreased by 0.23 and 0.46 logs, respectively, after composting. The RAs of 10/11 ARGs decreased in CK, whereas they all decreased in T3. The reduction in the RA of the total mobile genetic elements (MGEs) was 1.26 times higher in T3 compared with CK after composting. The bacterial community accounted for 47.93% of the variation in the abundances of ARGs. Network analysis indicated that ARGs and MGEs shared potential host bacteria (PHB), and T3 controlled the transmission of ARGs by reducing the abundances of PHB. Composting with 7.5% sepiolite is an effective strategy for reducing the risk of ARGs proliferating.

https://www.sciencedirect.com/science/article/abs/pii/S0960852422000566