摘要
      畜禽粪便作为抗生素抗性基因（ARGs）的主要来源，在田间用作肥料时，可能会进一步将ARGs从土壤转移到蔬菜中，从而对人类健康构成威胁。同时，粪肥投入和蔬菜种植也会影响土壤细菌群落，但这些对ARGs从土壤向蔬菜传播的影响仍然缺乏。在这里，通过盆栽试验，在粪肥改良的土壤中种植生菜和菊苣。研究了施肥改良土壤和收获时蔬菜根和叶中细菌群落、ARGs和intI1基因的分布。高通量测序分析表明，种植蔬菜对土壤细菌群落有显著影响，这在一定程度上解释了种植土壤中某些ARGs和intI1基因比对照土壤减少的原因。蔬菜和土壤中的ARGs相互关联。通过层次聚类分析，研究了根内生菌、叶内生菌和叶层细菌群落组成的差异。在根内生菌和土壤微生物之间发现了更高丰度的共享细菌类群，这可能导致根内生真菌中ARGs的检测频率相对较高。变形杆菌、放线菌、拟杆菌和厚壁菌门在植物内生菌和叶层微生物中占主导地位，并与ARGs密切相关。总之，我们的发现为细菌群落结构在ARGs从粪肥改良土壤传播到蔬菜中的作用提供了有价值的见解。
Abstract
Livestock manure, as a major source of antibiotic resistance genes (ARGs), could further transfer ARGs from soil to vegetables when it's used as fertilizer in field and then pose threat to human health. Meanwhile, manure inputs and vegetable planting also affect soil bacterial communities, but these effects on the transmission of ARGs from soil to vegetable is still lacking. Here, lettuce and endive were cultivated in manure-amended soils using pot experiment. The distribution of bacterial community, ARGs and intI1 gene were studied in manure-amended soil and vegetable roots and leaves at harvest. High-throughput sequencing analysis demonstrated that planting vegetables exerted significant effect on soil bacterial communities, which partly explained the decrease of certain ARGs and the intI1 gene in planted soil than in control soil. ARGs in vegetable and soil were interconnected. The bacterial community compositions among root endophyte, leaf endophyte, and phyllosphere were varied by Hierarchical clustering analysis. Higher abundance of shared bacterial taxa was found between root endophytes and soil microbes, which could lead to a relative higher detection frequency of ARGs in root endophyte. Proteobacteria, Actinobacteria, Bacteroidetes and Firmicutes were dominant in the plant endophyte and phyllosphere microbes and had intensive correlations with ARGs. Taken together, our findings provided valuable insights into the role of bacterial community structure in the dissemination of ARGs from manure-amended soil to vegetables.

https://www.sciencedirect.com/science/article/abs/pii/S004896972201556X