摘要
      食物垃圾是资源化利用的重要对象，也是抗生素抗性基因的来源。本研究调查了生物干燥与接种成熟堆肥（B&M）相结合对FW堆肥过程中堆肥效率、细菌群落演替及其与代谢功能的联系以及ARGs命运的影响。结果表明，B&M可以快速提高并保持Bacillaceae的相对丰度（66.59–94.44%）和堆肥温度（45.86–65.86℃），通过调节微生物碳水化合物（14.02–15.31%）和氨基酸代谢（10.33–12.47%），使FW堆肥在短时间内达到最终成熟。网络分析表明，高温可以有效抑制ARGs和整合子的潜在细菌宿主，包括乳酸杆菌科、肠杆菌科、，明串珠菌科和棒状杆菌科在堆肥的前两天。因此，B&M显著降低了几乎所有ARG的绝对丰度（72.09–99.47%）和相对丰度（0.31–2.44 log），尤其是ermB、tetM、blaCTX-M和blaOXA。本研究加深了对B&M工艺用于FW堆肥时ARGs变异、演替和细菌群落代谢功能的了解，为降低ARGs的传播风险和FW堆肥的成熟提供了一种很有前途的技术。
Abstract
Food waste (FW) is important object of resource utilization and source of antibiotic resistance genes (ARGs). This study investigated the effects of biodrying combined with inoculating mature compost (B&M) on the composting efficiency, succession of bacterial communities and their links with metabolism functions as well as the fate of ARGs during FW composting. The results showed that B&M could rapidly raise and maintain high relative abundance of Bacillaceae (66.59–94.44%) as well as composting temperature (45.86–65.86 ℃), so as to achieve the final maturity of FW composting in a short time by regulating microbial carbohydrate (14.02–15.31%) and amino acid metabolism (10.33–12.47%). Network analysis demonstrated that high temperature could effectively inhibit the proliferation and spread of potential bacterial hosts of ARGs and integrons including Lactobacillaceae, Enterobacteriaceae, Leuconostocaceae and Corynebacteriaceae during the first two days of composting. As a result, B&M significantly reduced the absolute (72.09–99.47%) and relative abundances (0.31–2.44 logs) of nearly all ARGs especially ermB, tetM, blaCTX-M and blaOXA. Present study deepened the knowledge of ARGs variation, succession and metabolism functions of bacterial communities when B&M processes were used for FW composting, suggesting a promising technology for reducing the transmission risk of ARGs and reaching maturity of FW composting.

https://www.sciencedirect.com/science/article/abs/pii/S0304389421028843