摘要
      以过硫酸盐（PS）为基础，研究了废活性污泥（WAS）发酵过程中抗生素抗性基因（ARGs）的发生情况。PS/Fe治疗可显著降低多药（mexP）、大环内酯（blaOXA-129）、四环素（tetB）、磺酰胺（sul1）和万古霉素（vanRG）型ARGs。机理研究表明，PS/Fe具有氧化潜力，对WAS发酵具有破坏性影响。首先，PS/Fe促进细胞结构损伤，从而促进ARGs从潜在宿主中释放。共现网络分析表明，Fe/PS抑制了潜在宿主细菌的增殖。此外，PS/Fe处理诱导参与ARGs传播相关途径的某些功能基因丰度降低。最后，变异划分分析表明，微生物群落结构对ARGs命运的影响比物理化学因素（即pH和ORP）和基因表达（即双组分系统）更为重要。这项工作对WAS发酵过程中用于确定ARGs命运的关键因素有了更深入的了解。
Abstract
The occurrence of antibiotic resistance genes (ARGs) in waste activated sludge (WAS) fermentation was investigated with persulfate (PS)-based treatment. ARGs affiliated with multidrug (mexP), macrolide (blaOXA-129), tetracycline (tetB), sulfonamide (sul1), and vancomycin (vanRG) types were significantly decreased by PS/Fe treatment. Mechanistic investigations revealed that PS/Fe possessed oxidating potential and exhibited devastating effects on WAS fermentation. First, PS/Fe promoted cell structure damage, which facilitated ARGs release from potential hosts. A co-occurrence network analysis indicated that Fe/PS suppressed the proliferation of potential host bacteria. In addition, the PS/Fe treatment induced the decreased abundance of certain functional genes involved in pathways associated with ARGs dissemination. Finally, variation partitioning analysis demonstrated that the microbial community structure exhibited more vital effects on ARGs fates than physicochemical factors (i.e., pH and ORP) and gene expression (i.e., two-component system). This work provided a deeper understanding of the critical factors used to determine ARGs fates during WAS fermentation.

https://www.sciencedirect.com/science/article/abs/pii/S0960852421018162