摘要
      先前的研究表明，通过热活化过氧二硫酸盐（TAP）预处理可以有效去除螺旋霉素发酵残渣（SFR）中残留的未提取抗生素，表明其生物降解性得到了提高。本研究旨在研究TAP预处理对SFR堆肥过程中细菌群落演替和抗生素抗性基因命运的影响。结果表明，TAP预处理提高了堆肥温度，促进了有机物的分解。此外，TAP预处理可以增加细菌α多样性，并显著降低堆肥后ARGs（1.13–1.75倍）和移动遗传元素（MGE）（1.13–1.32倍）的相对丰度。TAP预处理的SFR堆肥可以减少细菌群落中ARGs和MGE的富集，尤其是ermB的rRNA甲基化酶基因（4-142倍）。冗余分析表明，放线菌门、拟杆菌门、变形菌门和MGEs介导的水平基因转移（intI1）与ARGs的变化呈正相关（占97.4%）。网络分析表明，厚壁菌门是ARGs和MGEs的主要细菌宿主。这些发现表明，TAP预处理结合堆肥是一种很有前途的SFR安全处理和处置策略，可以减少ARGs的增殖和转移。
Abstract
Previous research has been demonstrated that the residual unextracted antibiotics in spiramycin fermentation residue (SFR) could be efficiently removed by thermally activated peroxodisulfate (TAP) pretreatment, indicating the improvement of biodegradability. This study aimed to investigate the effect of TAP pretreatment on the succession of bacterial community and fate of antibiotic resistance genes (ARGs) during SFR composting. Results indicated that TAP pretreatment increased the composting temperature and promoted the decomposition of organic matters. Furthermore, TAP pretreatment could increase bacterial alpha diversity and significantly reduce the relative abundance of ARGs (1.13–1.75 times) and mobile genetic elements (MGEs) (1.13–1.32 times) after composting. The compost of pretreated SFR by TAP could reduce the enrichment of ARGs and MGEs in the bacterial community, especially the rRNA methylase genes of ermB (4–142-folds). Redundancy analysis showed that Actinobacteria, Bacteroidetes, Proteobacteria and horizontal gene transfer mediated by MGEs (intI1) was positively related to the changes in ARGs (accounted for 97.4%). Network analysis showed that Firmicutes was the main bacterial hosts of ARGs and MGEs. These findings demonstrated that TAP pretreatment combined composting was a promising strategy for SFR safe treatment and disposal that could reduce the proliferation and transfer of ARGs.

https://www.sciencedirect.com/science/article/abs/pii/S030438942102255X