摘要
本研究旨在探讨堆肥过程中携带抗生素抗性基因（ARGs）的微生物的演替，以及重金属物种形成与ARGs通过类芬顿反应的关系。结果表明，Cu和Ni的钝化作用更为突出，类Fenton反应特别促进了Zn、Ni和Mn的钝化。堆肥过程诱导了大环内酯类链菌素（MLS）、氨基糖苷类和四环素抗性基因的去除，但杆菌肽抗性基因的相对丰度增加。此外，变形菌门、厚壁菌门、放线菌门和拟杆菌门是ARGs的主要携带者和传播者，Fenton样反应提高了变形菌门对杆菌肽、四环素和氨基糖苷类抗性基因的贡献程度。冗余分析表明，重金属的钝化有助于四环素、MLS和氨基糖苷类抗性基因的去除。总之，类芬顿反应促进了Zn、Ni和Mn的钝化，并控制了堆肥中杆菌肽抗性基因的丰度。
Abstract
This study aims to explore the succession of microbes carrying antibiotic resistance genes (ARGs), the relationship between heavy metal speciation and ARGs via Fenton-like reaction during composting. The results indicated that the passivation of Cu and Ni was more prominent, and the Fenton-like reaction promoted exceptionally the passivation of Zn, Ni and Mn. The removals of macrolides-lincosamids-streptogramins (MLS), aminoglycoside and tetracycline resistance genes were induced with the composting process, but the relative abundance of bacitracin resistance genes increased. Additionally, Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes were main carriers and disseminators of ARGs, and the Fenton-like reaction improved the contribution degree of Proteobacteria to bacitracin, tetracycline and aminoglycoside resistance genes. Redundancy analysis revealed the passivation of heavy metal contributed to the removal of tetracycline, MLS and aminoglycoside resistance genes. Conclusively, the Fenton-like reaction promoted the passivation of Zn, Ni and Mn, and controlled the abundance of bacitracin resistance genes in composting.

https://www.sciencedirect.com/science/article/abs/pii/S0960852422008057