摘要
      本研究研究了微藻生长对藻类-细菌颗粒污泥（ABGS）系统中抗生素去除和抗生素抗性基因（ARGs）/ARGs宿主菌衰减的影响。在四环素（TC）和磺胺嘧啶（SDZ）混合物（2–4 mg/L）的存在下，微藻可以在细菌颗粒污泥（BGS）上生长形成ABGS，叶绿素a含量达到7.68–8.13 mg/g-VSS。ABGS对TC和SDZ的去除率分别高达79.0%和94.0%，比BGS高4.3–5.0%。宏基因组分析表明，BGS中TC/SDZ相关ARGs和移动遗传元件（MGE）的相对丰度分别比ABGS高56.1%和22.1%。从这些颗粒中总共检测到26个ARGs，并确定它们与46种宿主细菌有关。26个ARG中的13个和46个主机中的13个中分别是共享的ARG和主机。BGS中宿主细菌的总相对丰度比ABGS中高30.8%。Scenedesmus和小球藻是可能降低ARGs宿主多样性的主要微藻。总的来说，ABGS是一种很有前途的含抗生素废水处理生物技术。
Abstract
This study investigated the effects of microalgae growth on antibiotic removal and the attenuation of antibiotic resistance genes (ARGs)/ARGs host bacteria in algal-bacterial granular sludge (ABGS) system. In the presence of tetracycline (TC) and sulfadiazine (SDZ) mixture (2–4 mg/L), microalgae could grow on bacterial granular sludge (BGS) to form ABGS, with a chlorophyll-a content of 7.68–8.13 mg/g-VSS being achieved. The removal efficiencies of TC and SDZ by ABGS were as high as 79.0 % and 94.0 %, which were 4.3–5.0 % higher than those by BGS. Metagenomic analysis indicated that the relative abundances of TC/SDZ- related ARGs and mobile genetic elements (MGEs) in BGS were 56.1 % and 22.1 % higher than those in ABGS. A total of 26 ARGs were detected from the granules, and they were identified to associate with 46 host bacteria. 13 out of 26 ARGs and 13 out of 46 hosts were shared ARGs and hosts, respectively. The total relative abundance of host bacteria in BGS was 30.8 % higher than that in ABGS. Scenedesmus and Chlorella were the dominant microalgae that may reduce the diversity of ARGs hosts. Overall, ABGS is a promising biotechnology for antibiotic-containing wastewater treatment.

https://www.sciencedirect.com/science/article/abs/pii/S0304389422010767