摘要
      本研究评估了青霉素G（PENG）对鸡粪堆肥过程中细菌群落命运和β-内酰胺酶抗生素抗性基因（ARGs）的影响，以阐明青霉素G在ARGs行为中的作用。结果表明，PENG使9种ARGs和4种可移动遗传元件（MGE）的总绝对丰度显著增加（P<0.05）。数十种ARGs.潜在宿主主要隶属于厚壁菌门、变形菌门和放线菌门。同时，较高浓度的PENG显著增加了群体感应（QS）中luxI和luxS的丰度（P<0.05），这增强了种间/种内基因“交流”的频率。冗余分析和结构方程建模进一步表明，QS在通过MGE介导的ARGs的水平基因转移中具有强大的调节作用。这些结果为PENG改性好氧堆肥中ARGs的繁殖机制提供了新的见解。
Abstract
In this study, the effects of penicillin G (PENG) on the fate of bacterial communities and β-lactamase antibiotic resistance genes (ARGs) during chicken manure composting were assessed, to illustrate the roles of PENG in ARGs behavior. The results showed that the total absolute abundances of 9 ARGs and 4 mobile genetic elements (MGEs) was significantly increased by PENG (P < 0.05). Dozens of potential hosts for ARGs were predominantly affiliated with Firmicutes, Proteobacteria, and Actinobacteria. Meanwhile, the higher concentration of PENG significantly increased the abundance of luxI and luxS in quorum sensing (QS) (P < 0.05), which enhanced the frequency of inter/intraspecific gene “communication.” Redundancy analysis and structural equation modeling further revealed that QS had a strong regulatory role in horizontal gene transfer of ARGs mediated via MGEs. These results provide new insight into the mechanism of ARGs propagation in aerobic composting modified by PENG.

https://www.sciencedirect.com/science/article/abs/pii/S0960852421017144