摘要
      本工作评估了中试规模的热水解预处理（THP）和随后的中温厌氧消化（AD）处理前后污水污泥中抗生素抗性基因（ARGs）和1类整合素基因的命运。研究的变量包括THP温度、原料类型和AD固体停留时间。进行实时聚合酶链式反应以定量原料、THP和AD流出物中的ARGs。结果表明，THP显著降低了大多数ARG的绝对丰度（t检验，p＜0.05），降低幅度在0.03至3.09 log单位之间。观察到ARGs在随后的AD中的反弹效应，并与测试变量相关；较短的固体停留时间（10天）和较高的THP温度（165℃）可以显著降低AD出水中的ARGs。这些发现为THP和AD对控制ARG从污水污泥扩散到环境的影响提供了参考。
Abstract
This work evaluated the fate of antibiotic resistance genes (ARGs) and class 1 integron gene in sewage sludge before and after pilot-scale thermal hydrolysis pretreatment (THP) and subsequent mesophilic anaerobic digestion (AD) treatment. Variables investigated include THP temperatures, feedstock types, and AD solids retention times. Real-time polymerase chain reaction was performed to quantify the ARGs in feedstocks, THP and AD effluent. Results show that THP significantly (t test, p < 0.05) reduced the absolute abundances of most ARGs, with the reduction ranging from 0.03 to 3.09 log units. Rebound effects of ARGs in the subsequent AD were observed and were relevant with tested variables; shorter solids retention time (10 days) and higher THP temperature (165 ℃) can significantly reduce ARGs in AD effluent. These findings provide references about the effects of the THP and AD on the control of ARG spread from sewage sludge to environments.

https://www.sciencedirect.com/science/article/abs/pii/S0960852422013761