摘要
      本研究调查了垃圾填埋场垃圾回收过程中气溶胶环境中细菌和抗生素抗性基因（ARGs）的分布和传播，包括挖掘现场、干燥场、筛选车间和办公区，以确定对人类和周围环境的潜在危害。结果表明，废物回收过程中释放的气溶胶对环境微生物的组成有显著影响，并可能通过扩散影响附近地区。对来自不同采样点的11种潜在人类致病菌（HPB）进行了观察，每个样本中的主要HPB属为不动杆菌属、泛菌属和克雷伯菌属，尤其是筛查车间中极高丰度的不动杆菌（50.43%）可能对工作人员构成威胁。采用液滴数字聚合酶链式反应检测了气溶胶中的4个抗性基因和3个移动遗传元件，并在筛选车间和办公区检测到了种类最多、浓度最高的抗性基因亚型。intI1与多达3种抗性基因亚型之间存在显著的正相关（P<0.05），而ARGs与细菌群落之间的相关性较弱。该研究测量并评估了废物循环过程中释放的气溶胶的风险，以指导必要的污染控制措施，特别是在随后实施此类项目时，在封闭和潮湿的环境中。
Abstract
This study investigated the distribution and transmission of bacteria and antibiotic resistance genes (ARGs) in aerosol environments during the process of waste recycling in the stock landfill, including the excavation site, drying field, screening workshop and office area, to identify potential hazards to people and the surrounding environment. Results revealed that aerosols released during waste recycling had a significant impact on composition of environmental microorganisms and may affect nearby areas by diffusion. Eleven potential human pathogenic bacteria (HPB) from different sampling sites were observed and the predominant HPB genera in each sample were Acinetobacter, Pantoea and Klebsiella, especially the extremely high abundance of Acinetobacter (50.43%) in the screening workshop may be a threat to the staff. Four resistance genes and three mobile genetic elements (MGEs) in aerosols were detected by droplet digital PCR, and the most kinds and high concentrations of resistance gene subtypes were detected in screening workshop and office area. A significant positive correlation was observed between intI1 and up to 3 resistance gene subtypes (P < 0.05), while the correlation between ARGs and bacterial communities was weak. The study measured and evaluated the resulting risk of aerosols released during waste cycling to guide the necessary pollution control measures especially in the closed and humid environment when subsequent such projects are implemented.

https://www.sciencedirect.com/science/article/abs/pii/S0021850222000623