发布者:抗性基因网 时间:2023-05-29 浏览量:199
摘要
堆肥茶是绿色农业中化学农药的替代品,但目前还没有关于堆肥茶中抗生素抗性基因(ARGs)的报道。本研究研究了畜禽粪便、污泥及其堆肥产品和液体发酵对各种堆肥茶的ARGs、移动遗传元素(MGEs)、金属抗性基因(MRGs)和抗菌性能的影响。结果表明,好氧液体发酵使鸡粪和污泥堆肥茶中的ARGs分别减少了65.93%和45.20%,使猪粪和牛粪堆肥茶中ARGs富集了8.57%和37.41%,使四种堆肥茶的MGE和MRG分别增加了1.25×10-5-5.53×10-3和2.03×10-5-2.03×10-3。堆肥产品和堆肥茶的四环素和磺酰胺抗性基因的相关系数分别为0.98和0.91。堆肥茶中富含的aadA2–02、sul2和tetX与MGE和MRG呈正相关。此外,液体发酵富集了四环素和万古霉素抗性基因的潜在宿主。在堆肥茶中,四环素抗性基因占总ARGs的62.7%。在四种堆肥茶中,产碱菌和芽孢杆菌富集了0.78–39.31%,其代谢产物具有较高的抗菌活性。ARGs的潜在宿主占四种堆肥茶中42.1%的细菌丰度
Abstract
Compost tea was alternatives of chemical pesticide for green agriculture, but there were no reports about antibiotic resistance genes (ARGs) in compost tea. This study investigated the effect of livestock manures, sewage sludge, their composting products and liquid fermentation on ARGs, mobile genetic elements (MGEs), metal resistance genes (MRGs) and antimicrobial properties of various compost tea. The results showed aerobic liquid fermentation reduced ARGs by 65.93 % and 45.20 % in the compost tea of chicken manure and sludge, enriched ARGs by 8.57 % and 37.41 % in the compost tea of pig manure and bovine manure, and increased MGEs and MRGs by 1.25 × 10-5-5.53 × 10-3 and 2.03 × 10-5-2.03 × 10-3 in the four compost tea. The correlation coefficient of tetracycline and sulfonamide resistance genes between compost product and compost tea were 0.98 and 0.91. aadA2–02, sul2 and tetX abundant in the compost tea were positively correlated with MGEs and MRGs. Furthermore, liquid fermentation enriched the potential host of tetracycline and vancomycin resistance genes. Tetracycline resistance genes occupied 62.7 % of total ARGs in the compost tea. Alcaligenes and Bacillus enriched by 0.78–39.31 % in the four compost tea, which metabolites had high antimicrobial activity. The potential host of ARGs accounted for 42.1 % bacteria abundance in the four compost tea
https://www.sciencedirect.com/science/article/abs/pii/S0304389422011888