摘要
      在本研究中，研究了纳米级零价铁（NZVI）对甲烷产生的促进和抑制作用以及抗生素抗性基因（ARGs）的减少的转折点。添加150 mmol/L的NZVI使甲烷产量最多增加23.8%，这是由于产生H2的化学反应和NZVI增强了种间直接电子转移（DIET）。NZVI350显著抑制了48.0%的甲烷生成，这可能与大量的活性氧（ROS）和过量的H2抑制微生物的功能有关。ARGs的命运与每日甲烷产量显著相关，这表明最终产生的甲烷产量越多，最终剩余的ARGs丰度就越少。ARGs的减少最多增加了61.0%，这归因于与NZVI腐蚀相关的空间位阻对垂直基因转移（VGT）和水平基因转移（HGT）的抑制。
Abstract
In this study, the turning point for nanoscale zero-valent iron’s (NZVI) promotion and inhibition effects of methane production coupled with the reduction of antibiotic resistance genes (ARGs) was investigated. Adding 150 mmol/L NZVI increased methane production by maximum of 23.8 %, which was due to the chemical reaction producing H2 and enhancement of direct interspecies electron transfer (DIET) by NZVI. NZVI350 dramatically repressed methane generation by 48.0 %, which might be associated with the large quantity of reactive oxygen species (ROS) and excessive H2 inhibiting the functioning of microorganisms. The fate of ARGs was significantly related to daily methane production, indicating that the more methane production finally generated, the less the abundance of ARGs at last left. The reduction of ARGs was enhanced by maximum of 61.0 %, which was attributed to the inhibition of vertical gene transfer (VGT) and horizontal gene transfer (HGT) caused by steric hindrance associated with NZVI corrosion.

https://www.sciencedirect.com/science/article/abs/pii/S0960852422009646