摘要
      黑土是作物生产和粮食安全最重要的土壤资源之一。在中国，它们主要分布在东北地区。为了调查土壤抗生素抗性分布模式和监测土壤质量，我们随机选择了中国东北的9块玉米田，并在高通量方法和网络分析的基础上分析了抗生素抗性基因（ARG）的分布和共现模式。检测到高遗传多样性（136个独特基因）和低ARG丰度（10−5–10−2拷贝/16S rRNA基因拷贝），ARG之间的相互作用相对较少。I型整合子基因在土壤中普遍存在，并与ARG呈正相关，这可能会增加ARG传播的风险。大多数ARGs与微生物密切相关。此外，一些ARG与抗生素、营养素和金属元素显著相关。ARGs的产生和传播很可能是由可移动遗传元件（MGE）和细菌介导的，受到环境条件的影响。这些结果为土壤抗性中广泛的共生模式提供了见解。
Abstract
Black soils (Mollisols) are among the most important soil resources for crop production and food security. In China, they are mainly distributed in the northeastern region. To investigate soil antibiotic resistance distribution patterns and monitor soil quality, we randomly chose nine corn fields in Northeast China and analyzed the antibiotic resistance gene (ARG) distribution and co-occurrence patterns on the basis of high-throughput approaches and network analyses. High genetic diversity (136 unique genes) and low ARG abundances (10−5–10−2 copies/16S rRNA gene copy) were detected, with relatively few interactions among ARGs. Type I integron genes were prevalent in the soil and were positively correlated with ARGs, which may increase the risk of ARG transmission. Most ARGs were strongly associated with microorganisms. Moreover, several ARGs were significantly correlated with antibiotics, nutrients, and metal elements. The generation and dissemination of ARGs, which were most likely mediated by mobile genetic elements (MGEs) and bacteria, were affected by environmental conditions. These results provide insights into the widespread co-occurrence patterns in soil resistomes.

https://www.sciencedirect.com/science/article/abs/pii/S0301479722012130