摘要
      三氯生（TCS）和聚氯乙烯微塑料（PVC-MPs）都被应用于个人护理产品中，这些产品可以同时进入废水。然而，在部分反硝化（PD）系统中，PVC MP对TCS去除和抗生素抗性基因（ARGs）繁殖的影响尚不清楚。在本研究中，研究了PVC MPs对不同碳源PD系统处理含TCS废水的PD性能、TCS去除、微生物群落和ARGs的影响。TCS能抑制反硝化作用，但R1醋酸酯对TCS的抗性强于R2甲醇。PVC MPs的加入可以促进NO2-N在R1乙酸盐和R2甲醇中转化为N2的脱氮过程。此外，PVC MP有利于TCS的降解。TCS的主要去除途径是在R1乙酸盐中降解，而在R2甲醇中吸附。PVC-MPs可以减少ARGs在R1乙酸盐中的增殖，但增加ARGs对R2甲醇的增殖。intI1或intI3可能主要负责ARGs通过水平基因转移的繁殖。此外，ARGs的潜在宿主是R1乙酸盐中的竞争性念珠菌和Defluviicoccus，而R2甲醇中的是嗜皮肤菌。本研究有助于理解PVC MP对处理含TCS废水的PD系统的影响。
Abstract
Both Triclosan (TCS) and polyvinyl chloride microplastics (PVC MPs) were applied in personal care products, which can simultaneously enter into wastewater. However, the effects of PVC MPs on TCS removal and antibiotic resistance genes (ARGs) propagation in partial denitrification (PD) systems were unknown. In this study, the impacts of PVC MPs on PD performance, TCS removal, microbial community and ARGs in different carbon source PD systems of treating wastewater containing TCS were investigated. TCS could inhibit denitrification, but R1-acetate had stronger resistant to TCS than R2-methanol. The addition of PVC MPs could promote denitrification process of the transformation of NO2--N to N2 in R1-acetate and R2-methanol. Moreover, PVC MPs could benefit TCS degradation. The main TCS removal pathway was degradation in R1-acetate while it was adsorption in R2-methanol. PVC MPs could decrease the propagation of ARGs in R1-acetate, but increase the proliferation of ARGs in R2-methanol. Both intI1 or intI3 might be mainly responsible for ARGs propagation through horizontal gene transfer. Moreover, the potential hosts of ARGs were Candidatus Competibacter and Defluviicoccus in R1-acetate, while it was Dermatophilus in R2-methanol. This study contributes to comprehend the impacts of PVC MPs on PD systems of treating wastewater containing TCS.

https://www.sciencedirect.com/science/article/abs/pii/S1385894721040432