摘要

      人们对水生环境中尸体分解引起的耐药性表示严重关切，这对水环境和人类健康构成了威胁。然而，对大容量自来水中的抗生素抗性基因及其在尸体腐烂过程中的时间稳定性的研究很少。在这里，高通量定量聚合酶链式反应（HT-qPCR）和扩增子测序被应用于在尸体分解过程中的第7天、第15天和第100天对含有50L自来水的实验组和对照组的ARGs和细菌群落进行分析。我们的结果表明，与对照组相比，实验组的大多数ARGs具有更高的丰度，与时间无关。一些ARGs的绝对丰度，如四环素和β-内酰胺酶，在尸体腐烂过程中甚至富集了259–413640倍。12种机会性病原体，尤其是伯克霍尔德菌、军团菌和盐单胞菌，随着分解的进行而显著增加。此外，网络分析表明，机会性病原体与ARGs显著相关。我们的研究结果强调，尸体腐烂增加了大容量饮用水中ARGs的丰度和多样性，与时间无关，同时表现出ARGs在时间上的持久性，从而揭示了动物尸体的有害影响。我们的研究也为尸体腐烂引起的水源水风险评估和管理提供了宝贵的建议。

Serious concerns have been raised regarding resistomes caused by corpse decomposition in the aquatic environment, which has posed threats to the water environment and human health. However, antibiotic resistance genes (ARGs) in large-volume tap water and their temporal stability during corpse decay are poorly explored. Here, high-throughput quantitative polymerase chain reaction (HT-qPCR) and amplicon sequencing were applied to profile ARGs and bacterial communities in experimental and control groups containing 50 L of tap water at 7th, 15th and 100th day during corpse decomposition. Our results suggested that most of the ARGs in experimental group had higher abundance compared with the control group independent of time. Some ARGs’ absolute abundance like tetracycline and beta-lactamase was even enriched by 259 – 413,640-folds during corpse decay. Twelve opportunistic pathogens, especially Burkholderia, Legionella and Halomonas, remarkably increased as decomposition proceeded. Furthermore, network analysis showed that opportunistic pathogens were significantly associated with ARGs. Our results emphasize that corpse decay increases the abundance and diversity of ARGs in large-volume drinking water independent of time while exhibiting temporal persistence of ARGs, thereby uncovering the harmful effects of animal cadavers. Our study also provides valuable suggestions for the risk assessment and management of source water caused by corpse decay.

https://www.sciencedirect.com/science/article/abs/pii/S096483052100202X