摘要
      揭示1类整合子在不同操作条件下的特性对于控制污泥厌氧消化过程中的抗生素抗性基因具有重要意义。本研究调查了厌氧污泥消化器中1类整合素和1类整合素携带的ARGs在25°C、35°C和55°C下的变化。结果显示，与25°C和35°C相比，55°C下具有长基因盒阵列的intI1丰度更低，I类整合素更少。在25°C和35°C的蒸煮器中观察到多抗性基因盒阵列。在所有以氨基糖苷类为主的消化器中，在1类整合子上检测到丰富的ARG。消化器中1类整合子在55°C时的ARGs丰度低于25°C和35°C时。在控制1类整合子携带的ARGs方面，嗜热AD优于中温AD。
Abstract
Revealing class 1 integron characteristics under different operating conditions is of great importance to control antibiotic resistance genes (ARGs) during sludge anaerobic digestion (AD). This study investigated the variations of class 1 integrons and the ARGs carried by class 1 integrons in anaerobic sludge digesters under 25 °C, 35 °C, and 55 °C. The results showed lower intI1 abundance and fewer class I integrons with long gene cassette arrays at 55 °C than at 25 °C and 35 °C. Multi-resistance gene cassette arrays were observed in the digesters at 25 °C and 35 °C. Abundant ARGs were detected on class 1 integrons in all digesters with aminoglycosides as the dominant class. The abundance of ARGs on class 1 integrons in digesters at 55 °C was lower than that at 25 °C and 35 °C. Thermophilic AD is better than mesophilic ones in the control of ARGs carried by class 1 integrons.

https://www.sciencedirect.com/science/article/abs/pii/S0960852422013384