摘要
      氯二甲酚（PCMX）是一种抗菌剂，在水环境中被广泛检测，对生物和生态具有毒性。在270d期间，研究了PCMX对三种硝化系统性能的影响，包括基于絮凝物的序批式反应器（FSBR）、基于好氧颗粒的SBR（AGSBR）和生物膜SBR（BSBR）。三个系统的硝化能力被PCMX抑制，但在驯化后恢复，PCMX使三个系统分别实现了10天、100天和35天的部分硝化。随着PCMX的增加，三个体系的胞外聚合物物质先增加后减少。AGSBR的颗粒结构可能有利于抗生素抗性基因（ARGs）的富集，并且在添加5.0mg/L PCMX的过程中，BSBR的几乎所有ARGs都减少了。微生物群落结果表明，红球菌作为潜在的降解菌在三个系统中不断富集。Piscinibacter被认为是潜在的抗生素耐药性细菌，在三个系统中与多种ARGs呈正相关。此外，在三个系统中，季铵化合物抗性基因与细菌具有多种正相关。本研究为PCMX的使用和治疗提供了一个新的视角。
Abstract
Chloroxylenol (PCMX), an antibacterial agent, has been widely detected in water environment and has toxic effects on biology and ecology. During 270 d, the influence of PCMX on the performance of three nitrification systems was investigated, including floc-based sequencing batch reactor (FSBR), aerobic granule-based SBR (AGSBR) and biofilm SBR (BSBR). The nitrification capability of three systems was inhibited by PCMX, but recovered after domestication, and PCMX made three systems realize partial nitrification for 10, 100 and 35 days, respectively. The extracellular polymeric substances of three systems increased first and then decreased with the increment of PCMX. The granular structure of AGSBR may be conducive to the enrichment of antibiotic resistance genes (ARGs), and almost all ARGs of BSBR were reduced during the addition of 5.0 mg/L PCMX. The microbial community results showed that Rhodococcus as potential degrading bacteria was continuously enriched in three systems. Piscinibacter was regarded as the potential antibiotic resistant bacteria, which was positively associated with multiple ARGs in three systems. Additionally, quaternary ammonium compounds resistance genes had a variety of positive correlations with bacteria in three systems. This study provided a new perspective for the usage and treatment of PCMX.

https://www.sciencedirect.com/science/article/abs/pii/S0304389422012584