摘要
      咸水养殖带来了许多经济效益，而水产养殖中的人为活动可能会导致抗生素抗性基因（ARGs）在咸水沉积物中传播。环境因素和微生物群落之间的复杂关系，以及它们在咸水养殖中ARGs传播中的作用，目前尚不清楚。本研究应用PCR和16S测序技术鉴定了咸水养殖沉积物中ARGs、1类整合素基因（intI1）和微生物群落的变异。ARGs在微咸水养殖沉积物中的分布与淡水养殖沉积物中相似，抗磺酰胺基因sul1是ARGs的指示基因。变形菌门（Proteobacteria）和厚壁菌门（Firmicutes。相关、网络和冗余分析结果表明，微咸水养殖沉积物中的微生物群落是功能驱动的。利用中性模型和变异划分分析对细菌群落的生态过程进行了验证。归一化随机比表明，池塘细菌群落以确定性为主，受水产养殖活动的影响。总氮和有机物影响ARGs的丰度，而变形杆菌和硫杆菌（p_Proteobacteria）是主要的抗生素抗性宿主。我们的研究深入了解了咸水养殖沉积物中ARGs的普遍性，并表明咸水养殖是ARGs库。
Abstract
Brackish water aquaculture has brought numerous economic benefits, whereas anthropogenic activities in aquaculture may cause the dissemination of antibiotic resistance genes (ARGs) in brackish water sediments. The intricate relationships between environmental factors and microbial communities as well as their role in ARGs dissemination in brackish water aquaculture remain unclear. This study applied PCR and 16S sequencing to identify the variations in ARGs, class 1 integron gene (intI1) and microbial communities in brackish water aquaculture sediment. The distribution of ARGs in brackish water aquaculture sediment was similar to that in freshwater aquaculture, and the sulfonamide resistance gene sul1 was the indicator of ARGs. Proteobacteria and Firmicutes were the dominant phyla, and Paenisporosarcina (p_ Firmicutes) was the dominant genus. The results of correlation, network and redundancy analysis indicated that the microbial community in the brackish water aquaculture sediment was function-driven. The neutral model and variation partitioning analysis were used to verify the ecological processes of the bacterial community. The normalized stochasticity ratio showed that pond bacteria community was dominated by determinacy, which was affected by aquaculture activities. The total nitrogen and organic matter influenced the abundance of ARGs, while Proteobacteria and Thiobacillus (p_Proteobacteria) were the key antibiotic-resistant hosts. Our study provides insight into the prevalence of ARGs in brackish water aquaculture sediments, and indicates that brackish water aquaculture is a reservoir of ARGs.

https://www.sciencedirect.com/science/article/abs/pii/S0048969722075775