摘要
      具有大比表面积的金属氧化物纳米颗粒（MONPs）有望与抗生素抗性基因（ARGs）结合，从而通过降低其浓度和动员来控制ARGs的污染。在这里，进行了吸附实验，发现α-Fe2O3 NP可以与水中的ARGs（携带tetM的质粒）化学结合，吸附速率为0.04 min−1，吸附容量为7.88 g/kg。将α-Fe2O3纳米颗粒混合到石英砂柱中，显著提高了对流入水中ARGs的截留去除率。1.0μg/mL ARGs在超纯水（25mL，5孔体积）中通过砂柱（有机玻璃，长8cm，内径1.4cm）和1g/kgα-Fe2O3 NPs的截留率是通过纯砂柱的1.73倍；随着α-Fe2O3 NPs添加量的增加，总的拦截率增加，当添加20g/kgα-Fe22O3 NPs时，拦截率达到68.8%。Na+（20mM）、Ca2+（20mm）和酸性条件（pH 4.0）共存可使1g/kgα-Fe2O3 NPs对ARGs的截留率进一步提高，分别从21.1%提高到86.2%、90.7%和96.2%。PO43−和腐殖酸在环境相关浓度下的存在不会显著影响ARGs的拦截。在PO43−和腐殖酸处理组中，去除率分别仅下降1.8%和0.1%。此外，掺入α-Fe2O3 NPs的砂柱对ARGs的截留去除率在实际地表水中（87.2%）甚至优于在超纯水中（21.1%）。这些发现为处理ARGs污染水提供了一种很有前途的方法。
Abstract
Metal oxide nanoparticles (MONPs) with a large specific surface area are expected to bind with antibiotic resistance genes (ARGs), thereby controlling ARGs' contamination by reducing their concentration and mobilization. Here, adsorption experiments were carried out and it was found that α-Fe2O3 NPs could chemically bind with ARGs (tetM-carrying plasmids) in water with an adsorption rate of 0.04 min−1 and an adsorption capacity of 7.88 g/kg. Mixing α-Fe2O3 NPs into quartz sand column markedly increased the interceptive removal of ARGs from inflow water. The interception rate of 1.0 μg/mL ARGs in ultrapure water (25 mL, 5 pore volumes) through the sand column (plexiglass, length 8 cm, internal diameter 1.4 cm) with 1 g/kg α-Fe2O3 NPs was 1.73 times of that through the pure sand column; the interception rate overall increased with increasing addition of α-Fe2O3 NPs, reaching 68.8% with 20 g/kg α-Fe2O3 NPs. Coexisting Na+ (20 mM), Ca2+ (20 mM), and acidic condition (pH 4.0) could further increase the interception rate of ARGs by 1 g/kg α-Fe2O3 NPs from 21.1% to 86.2%, 90.7%, and 96.2%, respectively. The presence of PO43− and humic acid at environmentally relevant concentrations would not significantly affect the interception of ARGs. In the treatment groups with PO43− and humic acid, the removal rate decreased by only 1.8% and 0.1%, respectively. In addition, the interceptive removal of ARGs by α-Fe2O3 NPs-incorporated sand column was even better in actual surface water samples (87.2%) than that in the ultrapure water (21.1%). The findings provide a promising approach to treat ARGs-polluted water.

https://www.sciencedirect.com/science/article/abs/pii/S0048969722023646