摘要
      监测抗生素耐药性基因（ARGs）对于“一个健康”应对抗生素耐药性危机的方法至关重要。有人提出，针叶树可以用作被动生物气溶胶采样器。在这里，使用针叶树作为生物气溶胶中ARGs的生物监测器被评估为概念验证。在加拿大魁北克省，人们从养猪场、村庄和森林周围的树木上采集针头。将针头匀浆并提取DNA。qPCR分析结果显示，生物量估计值在样本之间是一致的。与农场和村庄相比，森林地区的ARGs数量和数量明显较低，构成了一个独特的抗性群体。与之前的研究结果一致，最常见的ARGs是四环素类和磺酰胺类，它们是在农业活动附近发现的。尽管结果有限，但使用针叶树叶层作为被动生物气溶胶采样器有很大的潜力。这种方法代表了一种从点源远距离促进ARG监测的可行方法。
Abstract
Monitoring antibiotic resistance genes (ARGs) is vital to the One Health approach to tackling the antibiotic resistance crisis. It has been suggested that conifer needles can be used as passive bioaerosol samplers. Here, the use of conifer needles as biomonitors of ARGs in bioaerosols was assessed as a proof-of-concept. Needles were collected from trees surrounding pig farms, villages, and forest sites in Québec, Canada. Needles were homogenised and DNA was extracted. Results of qPCR analyses showed biomass estimates were consistent across samples. Number and quantity of ARGs was significantly lower in forest sites when compared to the farm and village, comprising a distinct resistome. Consistent with previous findings, the most common ARGs were tetracyclines and sulfonamides, which were found close to agricultural activities. Although results were limited, there is great potential for using the conifer phyllosphere as a passive bioaerosol sampler. This method represents an accessible way to promote ARG surveillance over long distances from point sources.

https://www.mdpi.com/2079-6382/11/7/907