摘要
      在绿色雨水基础设施（GSI）中，金属（多倍体）和土壤特征对细菌抗生素抗性基因（ARGs）的协同作用研究相对不足。在三个时间段内，评估了来自南加州六个GSI的表层土壤样本中选定的ARGs、1类整合子整合酶基因（intI1）、16S rRNA基因以及九种金属（多倍体）的生物可利用浓度和总浓度，以研究ARGs与土壤特征和共存金属（多倍体）之间的关系。相对基因丰度（sul1、sul2、tetW和intI1）、总金属（类似物）s（砷、铜、铅、钒和锌）和生物可利用金属（类似体）（砷）之间存在显著相关性（r=0.29–0.61，padj<0.05）。此外，土壤质地、有机质和GSI内的养分似乎与sul1、，和tetW（r=−0.57至0.59，padj<0.05）。与相关结果相比，多元回归模型在考虑土壤特征和金属（类）s（r=0.74，padj<0.001）的多重影响时，显著改善了GSI中ARGs的估计。总砷在所有相对基因丰度的回归模型中都是显著（正）相关的。这项工作为GSI ARG和共存金属（同源物）之间的共同依赖性提供了新的见解，表明需要对金属（同源体）影响的ARG增殖进行风险评估。
Abstract
The synergetic effects of metal(loid)s and soil characteristics on bacterial antibiotic resistance genes (ARGs) in green stormwater infrastructure (GSI) has been relatively understudied. Surface soil samples from six GSIs in Southern California over three time periods were assessed for selected ARGs, class 1 integron-integrase genes (intI1), 16S rRNA genes, and bioavailable and total concentrations of nine metal(loid)s, to investigate the relationships among ARGs, soil characteristics, and co-occurring metal(loid)s. Significant correlations existed among relative gene abundances (sul1, sul2, tetW, and intI1), total metal(loid)s (arsenic, copper, lead, vanadium, and zinc), and bioavailable metal(loid) (arsenic) (r = 0.29–0.61, padj < 0.05). Additionally, soil texture, organic matter, and nutrients within GSI appeared to be significantly correlated with relative gene abundances of sul1, sul2, and tetW (r = −0.57 to 0.59, padj < 0.05). Multiple regression models significantly improved the estimation of ARGs in GSI when considering multiple effects of soil characteristics and metal(loid)s (r = 0.74, padj < 0.001) compared to correlation results. Total arsenic was a significant (positive) correlate in all the regression models of relative gene abundances. This work provides new insights into co-dependencies between GSI ARGs and co-occurring metal(loid)s, indicating the need for risk assessment of metal(loid)-influenced ARG proliferation.

https://www.sciencedirect.com/science/article/abs/pii/S0304389421024377