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生物炭对抗生素菌丝体渣厌氧消化系统中抗生素抗性基因的影响

发布者:抗性基因网 时间:2023-06-06 浏览量:228

摘要
       为了解决抗生素菌丝体残渣(AMD)的处理和抗生素抗性基因(ARGs)的去除问题,本研究采用厌氧消化(AD)技术,添加生物炭(BC)和负载纳米零价铁的生物炭(nZVI-BC),以促进AMD的AD,提高ARGs的去除。结果表明,由于析氢腐蚀以及nZVI和BC的协同作用,nZVI BC在促进AD方面优于BC。此外,BC和nZVI-BC可以增强氧化应激反应,减少氨应激现象,从而显著降低aadA、ant(2〃)-Ⅰ、qacEdelta1和sul1的丰度。总之,nZVI BC的增强效果大于BC。nZVI-BC对上述四种ARGs的去除率分别提高了33%、9%、24%和11%。
Abstract
To address the problem of antibiotic mycelial dreg (AMD) treatment and removal of antibiotic resistance genes (ARGs), this study adopted anaerobic digestion (AD) technology, and added biochar (BC) and biochar loaded with nanosized zero-valent iron (nZVI-BC) to promote the AD of AMD and enhance the removal of ARGs. Results showed that nZVI-BC was better than BC in promoting AD due to the hydrogen evolution corrosion and the synergistic effect of nZVI and BC. In addition, BC and nZVI-BC can enhance the oxidative stress response and reduce ammonia stress phenomenon, which significantly reduces the abundance of aadA, ant(2″)-Ⅰ, qacEdelta1 and sul1. In conclusion, the enhance effect of nZVI-BC is greater than BC. The removal efficiency rates of nZVI-BC on the above-mentioned four ARGs were improved by 33%, 9%, 24% and 11%.

https://www.sciencedirect.com/science/article/abs/pii/S0960852422013852