摘要
客观的
确定骨关节炎（OA）或前交叉韧带（ACL）损伤患者体内和患者之间血清（sARGS）和滑液（sfARGS）中聚集蛋白聚糖酶产生的聚集蛋白聚糖ARGS新表位的生物学变异。
设计
作为临床试验的一部分，可从i）16名1年内8次早期OA受试者和ii）120名急性ACL损伤受试者获得血清和滑液的匹配样本，这些样本可从5年内6次就诊中的至少2次获得。我们使用内部免疫测定法对ARGS进行量化，并使用单因素方差分析进行统计分析。
后果
两组患者滑膜液中ARGS的变异性均高于血清中的变异性。OA受试者在患者内部和患者之间的变异性最低，并且随着时间的推移，无论是在血清还是在滑液中，变异程度或横截面平均值都没有变化。ACL损伤后，ARGS的浓度和变异性在损伤后立即达到最高，随后随着时间的推移，浓度和变异率都有所下降。在两个患者组中，sfARGS和sARGS在个体内部和个体之间都呈正相关（相关系数在0.16和0.20之间）。
结论
ARGS在血清中的生物学变异低于滑膜液，在OA中的生物学变化低于ACL损伤后。血清ARGS是衡量全身ARGS聚集蛋白聚糖总释放的指标，也是受影响关节内ARGS聚集因子聚糖释放的不良反映。
Abstract
Objective
To determine biological variation of the aggrecanase-generated aggrecan ARGS neoepitope in serum (sARGS) and synovial fluid (sfARGS) within and between patients with osteoarthritis (OA) or anterior cruciate ligament (ACL) injury.

Design
Matched samples of serum and synovial fluid were available, as parts of clinical trials, from i) 16 subjects with early-stage OA on 8 occasions over 1 year, and ii) 120 subjects with acute ACL injury with samples available from at least 2 of 6 visits over 5 years. We used an in-house immunoassay to quantify ARGS and one-way ANOVA for statistical analyses.

Results
Variability in ARGS was higher in synovial fluid than in serum in both patient groups. Subjects with OA had the lowest variability both within and between patients and showed no variation over time in the degree of variability or in the cross-sectional mean, neither in serum nor in synovial fluid. After ACL injury, the concentration and the variability of ARGS was highest immediately after injury, with a subsequent decline both in concentration and in variability with time. In both patient groups there was a positive correlation between sfARGS and sARGS both within and between individuals (correlation coefficients between 0.16 and 0.20).

Conclusions
The biological variation of ARGS is lower in serum than in synovial fluid, and lower in OA than after ACL injury. Serum ARGS is a measure of the total release of ARGS aggrecan from the whole body and a poor reflection of the release of ARGS aggrecan within the affected joint.

https://www.sciencedirect.com/science/article/pii/S2665913122000759