摘要
      生活垃圾填埋场被认为是抗生素抗性基因（ARGs）的主要来源，这些基因被认为是新出现的环境污染物。使用荧光定量PCR检测来确定垃圾渗滤液处理系统中ARGs的存在和丰度。最初在渗滤液影响物中检测到14个ARG。ARGs最丰富的是IntI1，其次是sulI、sulII、sulIII、tetC和tetW。ARGs的丰度在生物处理单元中增加，然后在超滤膜处理的出水中减少。出水中ARGs的绝对丰度比进水低1.05–1.94个数量级。各种ARGs与重金属，特别是Zn、Pb、Cu、Cd和As之间存在非常显著的强相关性，表明重金属与ARGs之间可能存在明显的共选择。尽管渗滤液处理工艺可以有效降低ARGs的丰度，但其减少ARGs物种数量的效果相对有限，这可能对接收渗滤液的城市污水厂和最终接收水体产生潜在的环境健康风险。
ABSTRACT
Domestic waste landfills have been regarded as the main source of antibiotic resistance genes (ARGs) which are considered to be emerging environmental contaminants. Fluorescent quantitative PCR detection was used to determine the occurrence and abundance of ARGs in a landfill leachate treatment system. Fourteen ARGs were detected initially in the leachate influents. The most abundant ARGs were IntI1, followed by sulI, sulII, sulIII, tetC and tetW. The abundance of ARGs increased in the biological treatment unit and then decreased in the effluent of ultrafiltration membrane treatment. The absolute abundance of ARGs in effluent was 1.05–1.94 orders of magnitude lower than that in influent. There was a very significant strong correlation between various ARGs and heavy metals, especially Zn, Pb, Cu, Cd and As, indicating that there may be obvious co-selection between heavy metals and ARGs. Although the leachate treatment process could effectively reduce the abundance of ARGs, its effect on reducing the number of species of ARGs was relatively limited, which may have potential environmental health risks to the urban sewage plant receiving leachate effluent and the final receiving water body.

https://www.tandfonline.com/doi/abs/10.1080/02757540.2022.2129624