摘要
      本研究研究了三种抗生素：土霉素（OTC）、环丙沙星（CIP）和克拉霉素（CLA）对四个测序间歇反应器（SBRs）中进行的厌氧氨氧化过程的抑制作用。每种抗生素的浓度为0.001 mg L−1，与城市污水处理厂中观察到的浓度相似。使用分批试验测量厌氧氨氧化过程的特异性厌氧氨氧化活性（SAA），使用高通量测序分析微生物群落结构，并通过qPCR测量功能基因和抗生素抗性基因（ARGs）丰度的变化。这些结果表明，没有一种抗生素显著影响厌氧氨氧化过程中的氮去除率（NRR）和特异性厌氧氨氧化活性（SAA）。对功能基因丰度变化的分析显示，在对照组中，抗生素对hzo的抑制作用显著，从3.88×10−6增加到1.26×10−5。几乎所有检测到的ARG的相对丰度都增加了（tetC和tetW靶向OTC，qnrB4和qnrS靶向CIP）。只有两种ARG（tetX靶向的OTC和mphA靶向的CLA）下降（分别从1.62×10−2和7.45×10−3降至3.74×10−4和9.3×10−5）。元分类学分析显示，在每个测试的反应器中，厌氧氨氧化菌属（Candidatus Brocadia，Candidats Jettenia）的相对丰度降低，而作为一种厌氧氨氧化微生物的硝化螺旋菌显著增加。本研究的目的是评估废水中三种常见抗生素（OTC、CIP、CLA）的微量浓度对厌氧氨氧化过程的影响。此外，CIP和CLA从未被研究过与厌氧氨氧化过程相关的问题。
Abstract
This study investigated the inhibitory effect of three antibiotics: oxytetracycline (OTC), ciprofloxacin (CIP), and clarithromycin (CLA) on the anammox process conducted in four sequencing batch reactors (SBRs). The concentration of each antibiotic was 0.001 mg L−1, similar to concentrations observed in municipal wastewater treatment plants. The specific anammox activity (SAA) of the anammox process was measured using a batch test, the microbial community structure was analyzed using high-throughput sequencing, and the variation of functional genes and antibiotic resistance genes (ARGs) abundance were measured by qPCR. These results indicated that none of the antibiotics significantly impacted nitrogen removal rate (NRR) in the anammox process and specific anammox activity (SAA). Analysis of the functional gene abundance variation showed significant inhibition of hzo by antibiotics while in control and increased from 3.88 × 10−6 to 1.26 × 10−5. The relative abundance of almost all detected ARGs increased (tetC and tetW targeted OTC, qnrB4 and qnrS targeted CIP). Only two ARGs (tetX targeted OTC and mphA targeted CLA) decreased (from 1.62 × 10−2 and 7.45 × 10−3 to 3.74 × 10−4 and 9.3 × 10−5, respectively). Metataxonomic analysis showed a decrease in the relative abundance of anammox bacteria genera (Candidatus Brocadia, Candidatus Jettenia) in each tested reactor, and a significant increase of Nitrospira, regarded as a comammox microorganism. The purpose of this study was to evaluate the effect of trace concentration of three commonly detected antibiotics (OTC, CIP, CLA) in wastewater on the anammox process. Furthermore, CIP and CLA have never been investigated relative to the anammox process.

https://www.sciencedirect.com/science/article/abs/pii/S2214714422000502