摘要
      从垂直（VGT）和水平基因转移（HGT）网络的角度，通过高通量qPCR（HT-qPCR）研究了氯化铁（FC）在厌氧消化（AD）系统中减少抗生素抗性基因（ARGs）的作用。尽管FC对猪粪AD中甲烷产生的影响有限，但四环素和MLSB抗性基因在最后被特异性降低，其中抗生素靶标保护的tetQ和抗生素靶标改变的ermF对降低的贡献最大。VGT和HGT网络都被分为三个模块，随着AD的出现，HGT网络的复杂性大大降低，其中HGT连接从683（模块III）减少到172（模块I），FC的添加可以进一步降低模块I中ARG主机的相对丰度，尽管微生物群落变化所反映的VGT对ARGs的动力学贡献最大（68.0%），但添加FC可以进一步降低HGT的贡献。这项研究为ARGs对AD系统中FC添加的反应的命运提供了一个新的视角。
Abstract
The role of ferric chloride (FC) on the reduction of antibiotic resistance genes (ARGs) in anaerobic digestion (AD) system was investigated from the perspective of vertical (VGT) and horizontal gene transfer (HGT) network through the high-throughput qPCR (HT-qPCR). Although FC showed limited impacts on methane production in AD of swine manure, the tetracycline and MLSB resistance genes were specifically reduced at the end, where tetQ of antibiotic target protection and ermF of antibiotic target alteration contributed the most to the reduction. Both VGT and HGT network were divided into three modules, and the complexity of HGT network was largely reduced along with AD, where the HGT connection was reduced from 683 (Module III) to 172 (Module I), and FC addition could further reduce the relative abundance of ARG hosts in Module I. The contribution of VGT and HGT to the changes of ARGs in AD was further deciphered, and although the VGT reflected by the changes of microbial community contributed the most to the dynamics of ARGs (68.0 %), the HGT contribution could further be reduced by the FC addition. This study provided a new perspective on the fate of ARGs response to the FC addition in the AD system.

https://www.sciencedirect.com/science/article/abs/pii/S0048969722041511